MDA5 抗体 (YA2399)

MDA5 Antibody (YA2399) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MDA5.

MDA5 是一种先天免疫受体，作为病毒核酸的胞质传感器，在感知病毒感染和激活一系列抗病毒反应（包括诱导 I 型干扰素和促炎细胞因子）中起主要作用。其配体包括 5' 端缺乏 2'-O-甲基化的 mRNA 和长链 dsRNA（长度 >1 kb）。配体结合后，MDA5 与线粒体抗病毒信号蛋白（MAVS/IPS1）结合，激活 IKK 相关激酶 TBK1 和 IKBKE，进而磷酸化干扰素调节因子 IRF3 和 IRF7，最终激活抗病毒免疫基因（如干扰素 IFN-alpha 和 IFN-beta）的转录。MDA5 负责检测小 RNA 病毒科（Picornaviridae）成员，如脑心肌炎病毒（EMCV）、门戈脑心肌炎病毒（ENMG）和鼻病毒（rhinovirus），也能检测 SARS-CoV-2 冠状病毒。此外，它还能识别登革病毒（DENV）、西尼罗河病毒（WNV）和呼肠孤病毒等其他病毒，并对含 dsDNA 基因组的病毒（如痘苗病毒）产生抗病毒信号。MDA5 通过识别核糖核酸酶 L（RNase L）在病毒感染期间产生的 RNA 代谢物，在放大先天免疫信号中起重要作用。它还可能通过增强自然杀伤细胞功能，并在多种肿瘤细胞系中参与生长抑制和凋亡过程。

Q9BYX4

Predicted band size: 117 kDa; Observed band size: 117 kDa

Affinity Chromatography

Non-conjugated

Unmodified

AB_3104754

Immunology

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

WB: 1:500-1:1000; IF: 1:50-1:200

• 
  Western blot analysis of extracts from THP-1(lane 2(20ug) ,Jurkat(lane 3(20ug) and HEK293(lane 4(20ug) using MDA5 Antibody (HY-P82654) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
• 
  Immunocytochemistry analysis of HepG2 cells labeling MDA5 with MDA5 Antibody (HY-P82654) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with MDA5 Antibody (HY-P82654) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunocytochemistry analysis of HepG2 cells labeling MDA5 with MDA5 Antibody (HY-P82654) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with MDA5 Antibody (HY-P82654) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

WB, ICC/IF

液体

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

A synthesized peptide derived from human MDA5