NQO1 抗体 (YA697)

NQO1 Antibody (YA697) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to NQO1.

NQO1 是一种含黄素 (flavin) 的醌还原酶 (quinone reductase)，能够利用 NADH 或 NADPH 作为电子供体，催化醌类 (quinones) 的两电子还原反应生成氢醌 (hydroquinones)。该酶通过乒乓动力学机制 (ping-pong kinetic mechanism) 依次将电子从 NAD(P)H 转移至黄素辅因子 (flavin cofactor)，再传递给醌类底物，从而避免半醌 (semiquinone) 和活性氧 (reactive oxygen species) 的生成 (By similarity)。其主要功能是通过醌解毒作用调节细胞氧化还原状态，可还原质膜氧化还原系统的组分（如辅酶 Q 和维生素醌类），生成具有抗氧化活性的氢醌形式；在此过程中还可能通过清除超氧化物 (superoxide) 防止氢醌氧化并促进其排泄。另一方面，该酶也能通过生成具有氧化还原活性的氢醌来激活醌类及其衍生物，使其获得与 DNA 交联的抗肿瘤潜力。此外，NQO1 作为 20S 蛋白酶体 (proteasome) 核心的看门蛋白，可在氧化应激条件下以 NADH 依赖性方式与肿瘤抑制蛋白 TP53 和 TP73 相互作用，抑制其通过 20S 蛋白酶体进行的泛素非依赖性降解。

Free

P15559

Predicted band size: 31 kDa; Observed band size: 31 kDa

Immunogen affinity purified.

Cytoplasm.

Non-conjugated

Unmodified

AB_3102380

Signal Transduction

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human, Rat

WB: 1:1000-1:5000; IHC-P: 1:50-1:100

• 
  Western blot analysis of extracts from A549 (lane 2(20μg), SiHa (lane 3(20μg), Hela (lane 4(20μg) using NQO1 Antibody (HY-P80250) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80250, 1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

WB, IHC-P

液体

Supplied in 1*PBS (pH7.4), 0.2% BSA and 50% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG2b

Endogenous

Synthetic peptide corresponding to Human NQO1.AA range:1-285.