NQO1 抗体 (YA261)

NQO1 Antibody (YA261) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NQO1.

NQO1 是一种含黄素 (flavin) 的醌还原酶 (quinone reductase)，能够利用 NADH 或 NADPH 作为电子供体，催化醌类 (quinones) 的两电子还原反应生成氢醌 (hydroquinones)。该酶通过乒乓动力学机制 (ping-pong kinetic mechanism) 依次将电子从 NAD(P)H 转移至黄素辅因子 (flavin cofactor)，再传递给醌类底物，从而避免半醌 (semiquinone) 和活性氧 (reactive oxygen species) 的生成 (By similarity)。其主要功能是通过醌解毒作用调节细胞氧化还原状态，可还原质膜氧化还原系统的组分（如辅酶 Q 和维生素醌类），生成具有抗氧化活性的氢醌形式；在此过程中还可能通过清除超氧化物 (superoxide) 防止氢醌氧化并促进其排泄。另一方面，该酶也能通过生成具有氧化还原活性的氢醌来激活醌类及其衍生物，使其获得与 DNA 交联的抗肿瘤潜力。此外，NQO1 作为 20S 蛋白酶体 (proteasome) 核心的看门蛋白，可在氧化应激条件下以 NADH 依赖性方式与肿瘤抑制蛋白 TP53 和 TP73 相互作用，抑制其通过 20S 蛋白酶体进行的泛素非依赖性降解。

Free

P15559

Predicted band size: 31 kDa; Observed band size: 31 kDa

Protein A affinity purified.

Cytoplasm.

Non-conjugated

Unmodified

AB_3102381

Signal Transduction

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse

WB: 1:1000-1:5000; ICC/IF: 1:50-1:200; FC: 1:50-1:100; IP: Use at an assay dependent concentration.

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  Immunocytochemistry analysis of HepG2 cells labeling NQO1 with NQO1 Antibody (HY-P80251) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with NQO1 Antibody (HY-P0251) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunocytochemistry analysis of Hela cells labeling NQO1 with NQO1 Antibody (HY-P80251) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with NQO1 Antibody (HY-P80251)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

WB, ICC/IF, IP, FC

液体

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human NQO1.AA range:1-49.