p21 抗体 (YA3460)

目录号: HY-P80774A: Data Sheet SDS COA 抗体使用指南
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p21 Antibody (YA3460) 是一个兔来源、无偶联标记、抗 p21 的 IgG 单克隆抗体。

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规格	价格	是否有货
10 μL	￥500	In-stock
50 μL	￥1300	In-stock
100 μL	￥2100	In-stock
250 μL		询价

or 大包装询价

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p21 Antibody (YA3460) 相关产品:

WB: 蛋白质免疫印迹;
IHC-P: 石蜡切片样本的免疫组织化学;
IHC-F: 冰冻切片样本的免疫组织化学;
ICC/IF: 细胞免疫荧光;
IF-Tissue: 组织免疫荧光;
mIHC: 多重荧光免疫组化;
IP: 免疫沉淀;
ChIP: 染色质免疫沉淀;
FC: 流式细胞术;
ELISA: 酶联免疫吸附试验

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描述

p21 Antibody (YA3460) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to p21.

宿主

Rabbit

克隆性

Monoclonal, Recombinant

分子量

Predicted band size: 18 kDa;

Observed band size: 21 kDa

请注意：因蛋白存在修饰或聚体等情况，以实测为准，预测仅为参考。

反应种属

Human, Mouse, Rat

蛋白数据库

P38936

基因 ID

1026 [NCBI]

免疫原

A synthesized peptide derived from human p21 aa115-164.

应用 & 推荐
稀释比例

应用	稀释比
WB WB: 蛋白质免疫印迹	1:500-1:1000
ICC/IF ICC/IF: 细胞免疫荧光	1:50-1:200

性状

液体

组分

Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

保存条件 & 期限

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

运输条件

Shipping with blue ice.

验证图片

ALL WB ICC

Western blot analysis of extracts from Hela (lane 2(20μg), U87 (lane 3(20μg) and RAW264.7 (lane 4(20μg) using p21 (HY-P80774A) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Immunocytochemistry analysis of MCF-7 cells labeling p21 with p21 antibody (HY-P80744A) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with p21 antibody (HY-P80744A) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
Immunocytochemistry analysis of MCF-7 cells labeling p21 with p21 antibody (HY-P80744A) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with p21 antibody (HY-P80744A) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

RRID

AB_3676571

反应种属数据库

Entrez Gene: 1026 Human ; 12575 Mouse ; 114851 Rat

SwissProt: P38936 Human ; P39689 Mouse ;

OMIM: 116899 Human

研究领域

Cell Biology

中文名

p21 抗体 (YA3460)

同用名

CDKN1A; CAP20; CDKN1; CIP1; MDA6; PIC1; SDI1; WAF1; Cyclin-dependent kinase inhibitor 1; CDK-interacting protein 1; Melanoma differentiation-associated protein 6; MDA-6; p21

文件资料

Select Batch:

Data Sheet (443 KB) SDS (251 KB)

COA (193 KB)

抗体使用指南 (1083 KB)

p21 Antibody (YA3460) 相关分类

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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沪(浦)应急管危经许[2021]201709(QFYS)

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p21 抗体 (YA3460)

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p21 抗体 (YA3460)

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