p38 alpha/MAPK14 抗体 (YA252) (YA252)

p38 Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 41 kDa, targeting to p38. It can be used for WB,IP assays with tag free, in the background of Human.

p38：该基因编码的蛋白质是 MAP 激酶家族的成员。MAP激酶作为多种生化信号的整合点，参与多种细胞过程，如增殖、分化、转录调控和发育。这种激酶被各种环境压力和促炎细胞因子激活。激活需要通过 MAP 激酶激酶 (MKK) 进行磷酸化，或者通过 MAP3K7IP1/TAB1 蛋白与该激酶的相互作用触发自身磷酸化。该激酶的底物包括转录调节因子 ATF2、MEF2C 和 MAX、细胞周期调节因子 CDC25B 和肿瘤抑制因子 p53，这表明该激酶在应激相关转录和细胞周期调节以及基因毒性应激反应中的作用。已经报道了编码不同同种型的该基因的四种可变剪接转录物变体。[由 RefSeq 提供，2008 年 7 月]

Free

Q16539

Predicted band size: 41 kDa; Observed band size: 41 kDa

affinity purified

Non-conjugated

Unmodified

AB_3102188

Signal Transduction

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

WB: 1:500-1:1,000 ;IP: 1:20

• 
  Western blot analysis of extracts from Hela(lane 2(20μg), A549 (lane 3(20μg) and U251(lane 4(20μg) using p38 (HY-P80777) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

WB, IP

液体

Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human p38.The exact sequence is proprietary to MCE.