p63 抗体 (YA1531)

目录号: HY-P81786: Data Sheet SDS COA 抗体使用指南
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p63 Antibody (YA1531) 是一个兔来源、无偶联标记、抗 p63 的 IgG 单克隆抗体。

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规格	价格	是否有货
10 μL	￥500	In-stock
50 μL	￥1375	In-stock
100 μL	￥2200	In-stock
250 μL		询价

or 大包装询价

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p63 Antibody (YA1531) 相关产品:

WB: 蛋白质免疫印迹;
IHC-P: 石蜡切片样本的免疫组织化学;
IHC-F: 冰冻切片样本的免疫组织化学;
ICC/IF: 细胞免疫荧光;
IF-Tissue: 组织免疫荧光;
mIHC: 多重荧光免疫组化;
IP: 免疫沉淀;
ChIP: 染色质免疫沉淀;
FC: 流式细胞术;
ELISA: 酶联免疫吸附试验

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描述

p63 Antibody (YA1531) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to p63.

宿主

Rabbit

克隆性

Recombinant, Monoclonal

分子量

Predicted band size: 77 kDa;

Observed band size: 77 kDa

请注意：因蛋白存在修饰或聚体等情况，以实测为准，预测仅为参考。

反应种属

Human, Mouse, Rat

蛋白数据库

Q9H3D4

基因 ID

8626 [NCBI]

免疫原

A synthesized peptide derived from human p63 aa1-230.

应用 & 推荐
稀释比例

应用	稀释比
WB WB: 蛋白质免疫印迹	1:500-1:1000
IHC-P IHC-P: 石蜡切片样本的免疫组织化学	1:50-1:100
ICC/IF ICC/IF: 细胞免疫荧光	1:50-1:200
FC FC: 流式细胞术	1:50-1:100

敏感性	Endogenous	纯度	Affinity Chromatography
偶联	Non-conjugated	修饰	Unmodified
同型	IgG

性状

液体

组分

Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

保存条件 & 期限

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

运输条件

Shipping with blue ice.

验证图片

ALL ICC FC

Immunocytochemistry analysis of A431 cells labeling p63 Antibody (HY-P81786) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with p63 Antibody (HY-P81786) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
Immunocytochemistry analysis of A431 cells labeling p63 Antibody (HY-P81786) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with p63 Antibody (HY-P81786) at 1/200 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
Flow cytometric analysis of 1X10^6 A431 cells labeling p63 (HY-P81786, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

RRID

AB_3103138

反应种属数据库

Entrez Gene: 8626 Human ; 22061 Mouse ; 246334 Rat

SwissProt: Q9H3D4 Human ; O88898 Mouse ; Q9JJP6 Rat

OMIM: 103285 Human

研究领域

Cell Biology

中文名

p63 抗体 (YA1531)

同用名

EEC3; KET; LMS; SHFM4; TA p63 alpha; TP63; p51; p73H; p73L; tumor protein p63

文件资料

Select Batch:

Data Sheet (443 KB) SDS (251 KB)

COA (193 KB)

抗体使用指南 (1083 KB)

p63 Antibody (YA1531) 相关分类

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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p63 抗体 (YA1531)

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p63 抗体 (YA1531)

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