Phospho-FAK (Tyr397) 抗体 (YA198)

Phospho-FAK (Tyr397) Antibody (YA198) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-FAK (Tyr397).

FAK (Focal Adhesion Kinase) 是一种非受体型蛋白酪氨酸激酶，在细胞迁移、黏附、铺展、肌动蛋白细胞骨架重组、黏着斑 (focal adhesions) 和细胞突起的形成与解聚、细胞周期进程、增殖及凋亡等过程中发挥关键调控作用。它对早期胚胎发育、胎盘形成、胚胎血管生成、心肌细胞迁移与增殖、心脏正常发育、轴突生长、神经元迁移、突触形成及神经系统发育至关重要。FAK 还参与成骨细胞分化和骨生成过程。其功能涉及整合素信号转导，以及多种生长因子受体、G 蛋白偶联受体 (GPCR)、EPHA2、netrin 受体和 LDL 受体下游的信号传递。FAK 与 SRC 家族成员形成多亚基信号复合物，通过磷酸化酪氨酸残基为支架蛋白、效应分子和底物提供结合位点，调控包括 PI3K-AKT1、MAPK/ERK 和 Rho 家族 GTPases 在内的多条通路。它还能磷酸化 NEDD9、SRC、ACTN1 等靶蛋白，并通过招募 MDM2 泛素连接酶调控 p53 的活性和降解。其截短亚型 FRNK 缺乏激酶结构域，可抑制 FAK 的磷酸化和信号传导。

Free

Q05397

Predicted band size: 119 kDa; Observed band size: 119 kDa

Protein A affinity purified.

Cytoplasm, Nucleus, Cell membrane, Cell junction.

Non-conjugated

Phosphorylated

AB_3102534

Cardiovascular

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse

WB: 1:500-1:1000; ICC/IF: 1:50-1:100

• 
  Western blot analysis of extracts from Hela (lane 2(20μg) and Hela (lane 3(40μg) using Phospho-FAK (Tyr397) (HY-P80460) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
• 
  Immunocytochemistry analysis of Hela cells labeling Phospho-FAK (Tyr397) with Phospho-FAK (Tyr397) Antibody (HY-P80460)at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-FAK (Tyr397) Antibody (HY-P80460) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunocytochemistry analysis of NIH/3T3 cells labeling Phospho-FAK (Tyr397) with Phospho-FAK (Tyr397) Antibody (HY-P80460) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-FAK (Tyr397) Antibody (HY-P80460) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

WB, ICC/IF

液体

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic phosphopeptide corresponding to residues surrounding Tyr397 of Human FAK.The exact sequence is proprietary to MCE.