Phospho-STAT3 (Ser727) 抗体 (YA147)

Phospho-STAT3 (Ser727) Antibody (YA147) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-STAT3 (Ser727).

STAT3 是一种信号转导器和转录激活剂，协调细胞对各种生长因子、白细胞介素和细胞因子的反应。激活后，STAT3 将共激活子招募到靶基因的启动子区域，从而介导多种细胞过程，例如增殖、分化和免疫反应调节。它对白细胞介素 6、KITLG/SCF 和其他生长因子的信号作出反应，促进一系列下游事件。此外，STAT3 在细胞周期调节、炎症反应调节和能量稳态中发挥作用。其功能延伸至影响细胞凋亡、黑皮质素产生和胰岛素分泌。蛋白质的激活涉及与相关家族成员的同二聚化或异二聚化。STAT3 与一系列分子相互作用，包括 IL31RA、NCOA1、PELP1、SIPAR、SOCS7、STATIP1 等，使其复杂地参与细胞信号传导通路。

Free

P40763

Predicted band size: 88 kDa; Observed band size: 88 kDa

Protein A affinity purified.

Cytoplasm, Nucleus.

Non-conjugated

Phosphorylated

AB_3102402

Signal Transduction

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Rat, Mouse

WB: 1:500-1:2000; ICC/IF: 1:50-1:200; IHC-P: 1:50-1:200; IP: Use at an assay dependent concentration.

• 
  Western blot analysis of extracts from Hela (lane 2(20μg) , NIH/3T3 (lane 3(20μg) , Jurkat (lane 4(20μg),using Phospho-STAT3 (Ser727) Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
• 
  Immunocytochemistry analysis of Heal cells labeling Phospho-STAT3 (Ser727) with Phospho-STAT3 (Ser727) Antibody (HY-P80281) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-STAT3 (Ser727)Antibody (HY-P80281) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunocytochemistry analysis of NIH3T3 cells labeling Phospho-STAT3 (Ser727) with Phospho-STAT3 (Ser727) Antibody (HY-P80281) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-STAT3 (Ser727)Antibody (HY-P80281)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunohistochemical analysis of paraffin-embedded mouse liver tissue using Phospho-STAT3 (Ser727) Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80281, 1:500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded mouse liver tissue using Phospho-STAT3 (Ser727) Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80281, 1:500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, ICC/IF, IHC-P, IP

液体

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic phosphopeptide corresponding to residues surrounding Ser727 of Human STAT3.AA range:701-750.