Phospho-Tau (Ser396) 抗体 (YA140)

Phospho-Tau (Ser396) Antibody (YA140) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-Tau (Ser396).

Tau 是一种促进微管 microtubule 组装和稳定性的蛋白质，可能参与神经元极性 neuronal polarity 的建立和维持。其 C 端结合轴突微管 axonal microtubules，而 N 端结合神经质膜成分 neural plasma membrane components，表明 Tau 作为连接蛋白 linker protein 在两者间发挥作用。TAU/MAPT 在神经元细胞中由中心体 centrosome 定义的细胞体域定位，决定了轴突极性 axonal polarity 的预设。较短的 Tau 异构体 short isoforms 赋予细胞骨架 cytoskeleton 可塑性，而较长的异构体 longer isoforms 可能更倾向于稳定细胞骨架。类似功能还包括：微管结合与神经元形态调控（by similar）。

Free

P10636

Predicted band size: 79 kDa;Observed band size: 70/130 kDa

Protein A affinity purified.

Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane, Microtubule, Secreted.

Non-conjugated

Phosphorylated

AB_3102553

Neuroscience

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse, Rat, Monkey, Pig

WB: 1:500-1:1,000 ;ICC/IF: 1:100-1:500 ;IHC-P: 1:50-1:200;IHC-F:1:200-1:500; IF-Tissue:1:100-1:500

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  Immunocytochemistry analysis of Hela cells labeling Phospho-Tau (Ser396) with Phospho-Tau (Ser396) Antibody (HY-P80480) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-Tau (Ser396) Antibody (HY-P80480)at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunocytochemistry analysis of Hela cells labeling Phospho-Tau (Ser396) with Phospho-Tau (Ser396) Antibody (HY-P80480) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-Tau (Ser396) Antibody (HY-P80480) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using Phospho-Tau Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using Phospho-Tau Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, IHC-P, IHC-F, ICC/IF, IF-Tissue

液体

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic phosphopeptide corresponding to residues surrounding Ser396 of Human Tau.The exact sequence is proprietary to MCE.