Phospho-YAP1 (Ser127) 抗体 (YA136)

Phospho-YAP1 (Ser127) Antibody (YA136) is a non-conjugated and Rabbit origined monoclonal antibody about 54 kDa, targeting to Phospho-YAP1 (Ser127). It can be used for WB,IHC-P assays with tag free, in the background of Human, Mouse, Rat.

YAP1：该基因编码 Hippo 信号通路的下游核效应子，参与发育、生长、修复和体内平衡。已知该基因作为该信号通路的转录调节因子在多种癌症的发生和发展中发挥作用，并可作为癌症治疗的潜在靶点。可变剪接导致编码不同异构体的多个转录变体。[由 RefSeq 提供，2013 年 8 月]

Free

P46937

Predicted band size: 54 kDa;Observed band size: 70-75 kDa

affinity purified

Non-conjugated

Phosphorylated

AB_3102234

Signal Transduction

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse, Rat

WB: 1:500-1:1,000 ;IHC: 1:50-1:100

• 
  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using Phospho-YAP1 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using Phospho-YAP1 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, IHC-P

液体

Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic phosphopeptide corresponding to residues surrounding Ser127 of Human YAP1.The exact sequence is proprietary to MCE.