RYK 抗体 (YA2481)

RYK Antibody (YA2481) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA2481), targeting RYK, with a predicted molecular weight of 68 kDa (observed band size: 72 kDa). RYK Antibody (YA2481) can be used for WB, IHC-F, IHC-P, ICC/IF, IP experiment in human background.

RYK 蛋白似乎与 FZD8 合作，作为 Wnt 蛋白（如 WNT1、WNT3、WNT3A 和 WNT5A）的潜在辅助受体。它涉及神经元分化、轴突引导、胼胝体建立和神经突生长等关键过程，强调了它在神经发育中的重要性。受到 WNT3 刺激后，受体在其跨膜区域发生 C 末端裂解，促进 C 末端细胞内产物从细胞质易位至细胞核。这种易位产物在介导神经元发育中发挥着至关重要的作用。RYK 在 Wnt 信号通路中的多方面参与强调了其作为协调神经分化和生长所必需的复杂细胞过程的关键参与者的潜力。进一步探索 RYK 的功能和调节机制可能会更深入地了解其对神经元发育的具体贡献。

P34925

Predicted band size: 68 kDa; Observed band size: 72 kDa

Affinity Purified

Non-conjugated

Unmodified

AB_3104834

-

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

WB: 1/500-1/1000;IHC: 1/50-1/100;IF: 1/50-1/200;IP: 1/20

• 
  Western blot analysis was performed on extracts from Jurkat (lane 1, 20 μg), HeLa (lane 2, 20 μg), A549 (lane 3, 20 μg), and U2OS (lane 4, 20 μg) using RYK Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

WB, IHC-F, IHC-P, ICC/IF, IP

液体

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

A synthetic peptide of human RYK