SOX9 抗体 (YA065)

SOX9 Antibody (YA065) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SOX9.

SOX9 是一种转录因子，在软骨细胞分化和骨骼发育中起关键作用。它特异性结合增强子和超级增强子中的 5'-ACAAAG-3' DNA 基序，促进软骨形成相关基因的表达，包括编码软骨基质的基因 COL2A1、COL4A2、COL9A1、COL11A2 和 ACAN，以及 SOX5 和 SOX6。类似功能 (By similarity) 还包括结合某些启动子区域，并在软骨细胞分化的连续步骤中发挥核心作用。它是前软骨凝聚 (precartilaginous condensation) 的绝对必需因子，这是软骨形成的第一步，期间骨骼前体细胞分化为前软骨细胞。与 SOX5 和 SOX6 共同作用，在第二步软骨形成中促使凝聚的前软骨细胞分化为早期软骨细胞。此外，它还调控生长板软骨细胞的肥大成熟并抑制其向成骨细胞分化：通过降低 β-catenin (CTNNB1) 信号和 RUNX2 表达，维持软骨细胞的柱状增殖，延迟前肥大状态并阻止成骨分化。类似功能 (By similarity) 还包括通过维持软骨细胞谱系命运和直接在上肥大细胞中激活 COL10A1 与 MEF2C，促进软骨细胞肥大。低脂水平是骨骼前体细胞向软骨分化的主要营养决定因素：当脂质水平低时，FOXO (FOXO1 和 FOXO3) 转录因子促进 SOX9 表达，从而诱导软骨分化并抑制脂肪酸氧化。类似功能 (By similarity) 还包括协助 (但不必须) 移除转录抑制的表观遗传标记并在软骨特异性基因上沉积活性启动子和增强子标记。与 Hedgehog 通路依赖的 GLI (GLI1 和 GLI3) 转录因子协同作用。除软骨发育外，SOX9 还调控上皮干/祖细胞的增殖和分化：在肺上皮分支形态发生中平衡增殖与分化并调节细胞外基质。类似功能 (By similarity) 还包括调控肾脏发育中的上皮分支。

Free

P48436

Predicted band size: 56 kDa;Observed band size: 70 kDa

Protein A affinity purified.

Nucleus.

Non-conjugated

Unmodified

AB_3102436

Neuroscience

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse

WB: 1:500; ICC/IF: 1:50-1:200; IHC-P: 1:50-1:200; FC: 1:50-1:100; IP: Use at an assay dependent concentration.

• 
  Immunocytochemistry analysis of MDA-MB-231 cells labeling SOX9 with SOX9 antibody (HY-P80335) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with SOX9 antibody (HY-P80335) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunocytochemistry analysis of MDA-MB-231cells labeling SOX9 with SOX9 antibody (HY-P80335) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with SOX9 antibody (HY-P80335) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunohistochemical analysis of paraffin-embedded Mouse liver tissue using SOX9 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80335, 1/200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded Mouse liver tissue using SOX9 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80335, 1/200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, IHC-P, ICC/IF, IP, FC, mIHC

液体

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human SOX9.AA range:140-340.