U1C 抗体 (YA2326)

U1C Antibody (YA2326) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to U1C.

U1C 是剪接体 U1 snRNP 的一个组成部分，对于识别 pre-mRNA 的 5' 剪接位点以及后续剪接体的组装至关重要。SNRPC/U1-C 直接参与组成型和调控型选择性剪接的初始 5' 剪接位点识别。它与 5' 剪接位点的相互作用似乎先于 pre-mRNA 和 U1 snRNA 之间的碱基配对。通过稳定 U1 snRNA 的 5' 端与 5' 剪接位点区域的碱基配对，促进 commitment 或早期 (E) 复合物的形成。by similar: 该蛋白在剪接位点识别和剪接体组装中具有保守功能。

P09234

Predicted band size: 17 kDa; Observed band size: 21 kDa

Affinity Purified

Non-conjugated

Unmodified

AB_3104682

Epigenetics and Nuclear Signaling

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Rat

WB: 1:500-1:1000; IHC: 1:50-1:100; IF: 1:50-1:200; IP: 1:20

• 
  Immunocytochemistry analysis of Hela cells labeling U1C with U1C Antibody (HY-P82581) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with U1C Antibody (HY-P82581) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunocytochemistry analysis of Hela cells labeling U1C with U1C Antibody (HY-P82581) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with U1C Antibody (HY-P82581) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

WB, IHC-F, IHC-P, ICC/IF, IP

液体

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

A synthetic peptide of human U1-C