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  3. C6 NBD Galactosylceramide

C6 NBD galactosylceramide 是半乳糖神经酰胺 (galactosylceramide) 的活性衍生物,带有荧光 C6 nitrobenzoxadiazole (C6 NBD) 标记。C6 NBD galactosylceramide 可作为中性 β-糖神经酰胺酶 (GCase) 的底物,研究半乳糖神经酰胺在细胞内的定位和代谢。

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C6 NBD Galactosylceramide Chemical Structure

C6 NBD Galactosylceramide Chemical Structure

CAS No. : 170212-26-7

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100 μg ¥6458
3 - 4 周

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  • 生物活性

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生物活性

C6 NBD galactosylceramide is an active derivative of galactosylceramide that is tagged with fluorescent C6 nitrobenzoxadiazole (C6 NBD). C6 NBD galactosylceramide can be used as a substrate for neutral β-glycosylceramidase (GCase) to study intracellular localization and metabolism of galactosylceramide (Ex=nm, Em=525)[1].

体外研究
(In Vitro)

C6-NBD-glucosylceramide (4 μM) is transported to the Golgi apparatus in HT29 cells[1].
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)[2].
Transcytosis of Exogenous C6-NBD-GaICer after Endocytosis:
1. C6-NBD-glucosylceramide is inserted at 10°C.
2. Cells are rinsed three times with cold HBSS' and incubated at 37°C in HBSS' to allow endocytosis.
3. After 10 min, the probe remaining on the cell surface is removed by two (apical) or three (basal) BSA washes for 20 min at 10°C.
4. One set of filters is used for lipid analysis to quantitate endocytosis. A second set of filters is further incubated for 0.5 or 1 h at 37°C in HBSS' + BSA, to assay for reappearance of intracellular C6-NBD-glucosylceramide on either cell surface.
5. The incubations are followed by a 10°C BSA wash, after which the NBD lipids from the combined apical media, basal media, and the cells were extracted into chloroform/methanol, analyzed by TLC, and quantitated.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

737.88

Formula

C36H59N5O11

CAS 号
Emission (Em)

525

Excitation (Ex)

489

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

纯度 & 产品资料
参考文献

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C6 NBD Galactosylceramide
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HY-D1575
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