1. Anti-infection Metabolic Enzyme/Protease
  2. Bacterial Endogenous Metabolite
  3. Cecropin P1, porcine

Cecropin P1, porcine 

目录号: HY-P2317 纯度: 99.87%
COA 产品使用指南

Cecropin P1, porcine 是一种抗菌 (antibacterial) 肽,可从猪小肠上部分离。Cecropin P1, porcine 对革兰氏阴性菌具有抗菌活性。Cecropin P1, porcine 具有抗病毒 (antiviral) 活性,可抑制 PRRSV 感染。

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Custom Peptide Synthesis

Cecropin P1, porcine Chemical Structure

Cecropin P1, porcine Chemical Structure

CAS No. : 125667-96-1

1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 价格 是否有货 数量
5 mg ¥2000
In-stock
10 mg ¥3200
In-stock
50 mg   询价  
100 mg   询价  

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Customer Review

Other Forms of Cecropin P1, porcine:

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Cecropin P1, porcine is an antibacterial peptide that can be isolated from the upper part of the small intestine of the pig. Cecropin P1, porcine shows antibacterial activity against Gram-negative bacteria. Cecropin P1, porcine shows antiviral activity and inhibits PRRSV infection[1][2].

体外研究
(In Vitro)

Cecropin P1, porcine (0-480 μg/mL, 36-96 h) markedly inhibits CH-1a infection and replication in Marc-145 cells[2].
Cecropin P1, porcine (0-480 μg/mL, 36 h) not only displays extracellular virucidal activity against PRRSV (porcine reproductive and respiratory syndrome virus), but also exerts a potent inhibitory effect when added either before, simultaneously with, or after viral inoculation[2].
Cecropin P1, porcine (480 μg/mL, 0-72 h) blocks CH-1a-induced apoptosis during the late phase of infection[2].
Cecropin P1, porcine (0-480 μg/mL, 0-4 h) inhibits viral particle release[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[2]

Cell Line: Marc-145 cells
Concentration: 160, 320, and 480 μg/mL
Incubation Time: 36, 48, 72, 96 h
Result: Significantly inhibited viral infection in a dose-dependent manner at 36 h postinfection. Inhibited CH-1a infection in Marc-145 cells with a 50% effective concentration (EC50) of 112 μg/mL. The 50% cytotoxic concentration (CC50) of Cecropin P1 for Marc-145 cells was estimated to be 719 μg/mL.

Western Blot Analysis[2]

Cell Line: Marc-145 cells
Concentration: 160, 320, and 480 μg/mL
Incubation Time: 36 h
Result: Significantly inhibited viral infection in a dose-dependent manner at 36 h postinfection. Significantly reduced the expression of the viral N protein when administered with either the pre-, co-, or posttreatment method.
体内研究
(In Vivo)

Cecropin P1 (1 mg/kg, IP, once) prevents bacterial growth, endotoxemia, and mortality in rats with septic shock[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

3338.86

Formula

C147H253N45O43

CAS 号
性状

固体

颜色

White to off-white

Sequence

Ser-Trp-Leu-Ser-Lys-Thr-Ala-Lys-Lys-Leu-Glu-Asn-Ser-Ala-Lys-Lys-Arg-Ile-Ser-Glu-Gly-Ile-Ala-Ile-Ala-Ile-Gln-Gly-Gly-Pro-Arg

Sequence Shortening

SWLSKTAKKLENSAKKRISEGIAIAIQGGPR

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Sealed storage, away from moisture and light, under nitrogen

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)

溶解性数据
In Vitro: 

H2O 中的溶解度 : 50 mg/mL (14.98 mM; 超声助溶)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 0.2995 mL 1.4975 mL 2.9950 mL
5 mM 0.0599 mL 0.2995 mL 0.5990 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

* 备注:如您选择水作为储备液,请稀释至工作液后,再用 0.22 μm 的滤膜过滤除菌后使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物溶解方案计算器
请输入动物实验的基本信息:

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

由于实验过程有损耗,建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
该产品水溶性佳,请具体参考实测 水 / PBS / Saline 中的溶解度数据。
您所需的储备液浓度超过该产品的实测溶解度,如有需要,请与 MCE 中国技术支持联系。
纯度 & 产品资料
参考文献

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 0.2995 mL 1.4975 mL 2.9950 mL 7.4876 mL
5 mM 0.0599 mL 0.2995 mL 0.5990 mL 1.4975 mL
10 mM 0.0300 mL 0.1498 mL 0.2995 mL 0.7488 mL

* 备注:如您选择水作为储备液,请稀释至工作液后,再用 0.22 μm 的滤膜过滤除菌后使用。

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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产品名称:
Cecropin P1, porcine
目录号:
HY-P2317
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