CO probe 1 

CO probe 1 (probe 2) is a highly efficient fluorescent CO probe (Ex=493 nm) with an allyl ether reaction site. In the presence of PdCl₂, CO reduces Pd²⁺ to Pd⁰, triggering a Tsuji-Trost reaction that removes the allyl protecting group, releases fluorescein, and generates a significant fluorescence signal. CO probe 1 has high selectivity, rapid response (fluorescence enhancement of 150 times within 20 minutes), and low cytotoxicity, and can be used for real-time imaging of CO in living cells. CO probe 1 may be used to study pathological mechanisms involving CO signaling regulation, such as inflammation, vascular disease, or cancer^[1].

CO probe 1 在活细胞中 CO 的成像步骤^[1]：
1. 细胞成像实验前，将细胞接种于 24 孔培养板中过夜。
2. 设置对照组和荧光组。对照组：活细胞与 CO probe 1 (1 μM) 在 37 °C 下孵育 30 分钟。荧光组：活细胞与 CO probe 1 和 PdCl₂ 的混合物 (各 1 μM) 在 37 °C 下孵育 30 分钟。
3. 以上两组孵育完成后，统一用 PBS 清洗三次后进行成像。
* 监测细胞外源 CO 时，将 HeLa 细胞用 CORM-3 (HY-100581) (分别为 1、5 和 10 μM) 在 37 °C 下预处理 30 分钟，再用 CO probe 1 和 PdCl₂ 的混合物 (各 1 μM) 在 37 °C 下孵育 30 分钟。用 PBS 缓冲液清洗细胞后再成像。
* 监测血红素刺激产生的 CO 时，其步骤与外源性 CO 的成像基本相同，但在细胞预处理中使用血红素 (100 μM) 代替 CORM 3 (HY-100581)。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

481.32

C₂₆H₁₈Cl₂O₅

1043865-37-7

固体

Light yellow to yellow

Room temperature in continental US; may vary elsewhere.

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

• [1]. Feng S, et al. Allyl Fluorescein Ethers as Promising Fluorescent Probes for Carbon Monoxide Imaging in Living Cells. Anal Chem. 2017 Mar 21;89(6):3754-3760.  [Content Brief]