2. Others Metabolic Enzyme/Protease
  3. Fluorescent Dye Liposome
  4. eGFP mRNA-LNP (500 ng/μL)

eGFP mRNA-LNP (500 ng/μL) 是一种含有 eGFP mRNA 的脂质纳米颗粒 (LNP)。eGFP mRNA-LNP 携带增强型绿色荧光蛋白 (eGFP)，进入细胞后会表达绿色荧光蛋白。eGFP 通常用作可通过荧光显微镜或流式细胞术检测的报告基因 (激发波长 = 488 nm; 发射波长 = 535/40 带通)。eGFP mRNA-LNP 可用于 RNA 递送、翻译效率和细胞活力。

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eGFP mRNA-LNP (500 ng/μL) Chemical Structure

eGFP mRNA-LNP (500 ng/μL) Chemical Structure

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500 μg ￥11200
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eGFP mRNA-LNP (500 ng/μL) 相关抗体

Top Publications Citing Use of Products

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

eGFP mRNA-LNP (500 ng/μL) is a lipid nanoparticle (LNP) containing eGFP mRNA. eGFP mRNA-LNP carries Enhanced Green Fluorescent Protein (eGFP), which will express green fluorescent protein after entering the cell. eGFP is commonly used as a reporter gene detectable by fluorescence microscopy or flow cytometry (excitation wavelength = 488 nm; emission wavelength = 535/40 bandpass). eGFP mRNA-LNP can be used for RNA delivery, translation efficiency and cell viability[1].

体外研究
(In Vitro)

eGFP mRNA-LNP (500 ng/μL) (1 μM，24 小时) 在 HeLa 细胞系中可使大量 mRNA 制剂翻译成 eGFP 蛋白，而巨噬细胞中的翻译量较低[2]。
eGFP mRNA-LNP (500 ng/μL) (1 μM，24 小时) 在库普弗细胞 (KC) 中产生微弱信号，而肝细胞在整个细胞中均可观察到 eGFP 的表达[2]。
eGFP mRNA-LNP (500 ng/μL) (0.25-1 μM，24-48 小时) 在 0.25 μM 浓度下表现出时间依赖性的 eGFP 转染，而在 1 μM 浓度下，肺泡上皮细胞和人原代肺成纤维细胞中时间差异不大[3]。
eGFP mRNA-LNP (500 ng/μL) (0.4 μM，48 小时) 在一半的细胞系表现出中等或较差的转染水平，而人类结肠上皮细胞 HCT116 和人肝细胞 HuH-7 细胞是高表达细胞[4]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

eGFP mRNA-LNP (500 ng/μL) 相关抗体:

Immunofluorescence[2]

Cell Line: Hepatocytes
Concentration: 1 μM
Incubation Time: 24 h
Result: Had a minor impact on the cell cycle progression of hepatocyte after transfection.
Did not affect the pattern of hepatocyte subsets.
Showed that big hepatocytes expressed the highest levels of eGFP.
体内研究
(In Vivo)

eGFP mRNA-LNP (500 ng/μL) (2 mg/kg，静脉注射，单剂量) 在注射的 16 小时后通过活体成像检测到 C57Bl/6J 小鼠肝细胞中的 eGFP 信号[2]。
eGFP mRNA-LNP (500 ng/μL) (3 mg/kg，静脉注射，单剂量) 可在 C57Bl/6J 小鼠的库普弗细胞 (KC) 和单核巨噬细胞 (MoMϕ) 中检测到显著的 eGFP 蛋白表达水平[2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: C57BL6J wild type mice[2]
Dosage: 2 mg/kg
Administration: Intravenous injection (i.v.), single dose
Result: Resulted in varied protein translation intensity between different hepatocytes, larger hepatocytes exhibited stronger signals than smaller ones.
Did not detect obvious signal in any other hepatic cell type.
Resulted in smaller and rounder KC compared to control mice, where the KC appeared to be elongated.
Animal Model: C57BL6J wild type mice[2]
Dosage: 3 mg/kg
Administration: Intravenous injection (i.v.), single dose
Result: Displayed a pronounced autofluorescence in HSC and hepatocytes of mice.
Showed significant amounts were taken up by hepatocytes and KC but not HSC.
性状

液体

颜色

Colorless to light yellow

运输条件

Shipping with dry ice.
储存方式

-80°C
纯度 & 产品资料
参考文献

eGFP mRNA-LNP (500 ng/μL) 相关分类

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

eGFP mRNA-LNP (500 ng/μL)Fluorescent DyeLiposomeLipid nanoparticlegreen fluorescent proteinreporter geneRNA deliveryInhibitorinhibitorinhibit

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eGFP mRNA-LNP (500 ng/μL)
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