Py-BODIPY-NHS ester 

BODIPY dye is a small molecule dye with strong ultraviolet absorption ability, its fluorescence peak is relatively sharp, and the quantum yield is high. They are relatively insensitive to the polarity and pH of the environment and are relatively stable under different physiological conditions^[1]. Due to its structural asymmetry, BODIPY derives a variety of structural products. BODIPY lipid droplet dyes can well pass through the cell membrane into the cell, and localize the neutral lipids in the cell to specifically stain the lipid droplets, which can be used for labeling of live cells and fixed cells^[2].

1.Py-BODIPY NHS ester 工作液的配制
1.1 制备储存液
用 382 μL 无水 DMSO 稀释 1mg Py-BODIPY NHS ester 去配制 10 mM 的储备液。
注：Py-BODIPY NHS ester 储存液建议分装后于 -20 ℃ 或 -80 ℃ 避光保存。
1.2 工作液的配制
用预热好的无血清细胞培养基或 PBS 稀释储存液，配制成 1-10 μM 的 Py-BODIPY NHS ester工作液。
注：请根据实际情况调整 Py-BODIPY NHS ester工作液浓度，且现用现配。
2. 细胞染色 (悬浮细胞)
2.1 离心收集细胞，加入 PBS 洗涤两次，每次 5 分钟。细胞密度在 1×106/mL。
2.2 加入 1 mL Py-BODIPY NHS ester 工作液，室温孵育 5-30 分钟。
2.3 400 g，离心 3-4 分钟，弃去上清。
2.4 加入 PBS 洗涤细胞两次，每次 5 分钟。
2.5 用 1 mL 无血清培养基或 PBS 重悬细胞后，使用荧光显微镜或流式细胞仪进行观察。
3. 细胞染色 (贴壁细胞)
3.1 将贴壁细胞培养于无菌盖玻片上。
3.2 从培养基中移出盖玻片，吸除多余培养基。
3.3 加入 100 μL 染料工作液，轻轻晃动使其完全覆盖细胞，孵育 5-30 分钟。
3.4 吸去染料工作液，用培养基洗 2-3 次，每次 5 分钟，使用荧光显微镜或流式细胞仪进行观察。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

426.18

C₂₀H₁₇BF₂N₄O₄

201998-61-0

固体

Brown to black

512

502

Room temperature in continental US; may vary elsewhere.

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

• [1]. Kamkaew A, et al. BODIPY dyes in photodynamic therapy. Chem Soc Rev. 2013 Jan 7;42(1):77-88.  [Content Brief]

  [2]. Vasta JD, et al. Quantitative, Wide-Spectrum Kinase Profiling in Live Cells for Assessing the Effect of CellularATP on Target Engagement. Cell Chem Biol. 2018 Feb 15;25(2):206-214.e11.  [Content Brief]

  [3]. Kang ZB, et al. Fluopack screening platform for unbiased cellular phenotype profiling. Sci Rep. 2020 Feb 7;10(1):2097.  [Content Brief]

  [4]. Robers MB, et al. Quantitative, Real-Time Measurements of Intracellular Target Engagement Using Energy Transfer. Methods Mol Biol. 2019;1888:45-71.  [Content Brief]

  [5]. Yuan H, et al. Fluorescent and radiolabeled triphenylphosphonium probes for imaging mitochondria. Chem Commun (Camb). 2013 Nov 14;49(88):10361-3.  [Content Brief]