1. MCE Kits
  2. Cell Biology
  3. Cell Analysis
  4. Cell Proliferation and Cytotoxicity
  5. Cytotoxicity LDH Assay Kit

Cytotoxicity LDH Assay Kit 

LDH 细胞毒性检测试剂盒

Cat. No.: HY-K1090
Manual SDS

MCE LDH Assay Kit 是通过分析释放到培养上清液中的 LDH 活性而测定细胞损伤的试剂盒。配合使用 CCK-8 (HY-K0301) 可获得死细胞、活细胞数据。

Cytotoxicity LDH Assay Kit

1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 价格 是否有货 数量
Free Sample (10T)   Apply now  
100 T ¥674 In-stock
500 T ¥1580 In-stock

* Please select Quantity before adding items.

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  • Description

  • Storage

  • Components

  • Documentation

Description
& Advantages

The loss of cell membrane integrity due to cell death or membrane damage results in release of certain soluble, cytosolic enzymes. Therefore, acute cytotoxicity can be measured by measuring activity of these enzymes released into the culture supernatant. Lactate dehydragenase (LDH) is a stable enzyme, present in all cell types, and rapidly released into the cell medium upon damage of the plasma membrane. LDH, therefore, is the most widely used marker in cytotoxicity study.

LDH oxidizes lactate to generate NADH, which then reacts with certain dye to generate yellow color. The intensity of the generated color correlates directly with the cell number lysed, which is indicative of cytotoxicity. LDH activity can be easily quantified by spectrophotometer or plate reader at 490 nm.

Fig 1. Schematic of MCE Cytotoxicity LDH Assay Kit.

MCE Cytotoxicity LDH Assay Kit can be directly added to cells to detect LDH activity in the culture supernatant (one-step, direct method). Or you can separate the culture supernatant from cell and use this kit to detect LDH activity (indirect method). The separated cells can be used for other experiments.

Fig 2. Detect LDH activity by direct and indirect method.

Publications
Storage

Stored at -20°C protecting from light, and is stable for up to 12 months.

Components
Components HY-K1090-100T HY-K1090-500T
Working Solution 5.5 mL 27.5 mL
Stop Solution 5.5 mL 27.5 mL
Lysis Solution 1.1 mL 5.5 mL
Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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产品名称:
LDH 细胞毒性检测试剂盒
目录号:
HY-K1090
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