2. 点击化学
  3. 炔烃
  4. JH295 hydrate

JH295 hydrate 

目录号: HY-116423A 纯度: ≥99.0%
COA 产品使用指南

摩尔计算器

JH295 hydrate 是一种有效，不可逆和选择性的 NIMA-related kinase 2 (Nek2) 抑制剂，IC50 为 770 nM。JH295 hydrate 通过 Cys22 的烷基化抑制细胞 Nek2。JH295 hydrate 对有丝分裂激酶 Cdk1，Aurora B 或 Plk1 没有活性，并且不会干扰双极纺锤体组件或纺锤体组件检查点。JH295 (hydrate) 是一种点击化学试剂。它含有 Alkyne 基团，可以和含有 Azide 基团的分子发生铜催化的叠氮-炔环加成反应 (CuAAc)。

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JH295 hydrate Chemical Structure

JH295 hydrate Chemical Structure

CAS No. : 2714087-26-8

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规格 价格 是否有货
1 mg ￥7000
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JH295 hydrate 的其他形式现货产品:

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Other Forms of JH295 hydrate:

JH295 hydrate 相关抗体

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  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

JH295 hydrate is a potent, irreversible and selective NIMA-related kinase 2 (Nek2) inhibitor with an IC50 of 770 nM. JH295 hydrate inhibits cellular Nek2 via alkylation of Cys22. JH295 hydrate is inactive against the mitotic kinases, Cdk1, Aurora B or Plk1, and does not perturb bipolar spindle assembly or the spindle assembly checkpoint[1]. JH295 (hydrate) is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.

IC50 & Target

IC50: 770 nM (Nek2)[1]
体外研究
(In Vitro)

JH295 (Compound 16; 0.08-20 μM; 45 minutes; RPMI7951 cells) treatment inhibits WT Nek2 in cells with an IC50 of ~1.3 μM, whereas it has little effect on the C22V mutant[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

JH295 hydrate 相关抗体:

Western Blot Analysis[1]

Cell Line: RPMI7951 cells
Concentration: 0.08 μM, 0.25 μM, 0.74 μM, 2.2 μM, 6.6 μM, 20 μM
Incubation Time: 45 minutes
Result: Inhibited WT Nek2 in cells with an IC50 of ~1.3 μM, whereas had little effect on the C22V mutant.
分子量

338.36

Formula

C18H18N4O3
CAS 号
性状

固体

颜色

Yellow to brown

运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
纯度 & 产品资料

纯度: ≥99.0%

COA (265 KB)

产品使用指南 (1538 KB)
参考文献

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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

JH2952714087-26-8JH 295JH-295OthersNek2centrosomalCys22propynamidecellularalkylationcell-permeableInhibitorinhibitorinhibit

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