MB-Buf 

MB-Buf is a conjugation of methylene blue (HY-14536) and bufalin (HY-N0877) . MB-Buf can targeting degrade GPX4 upon light activation. MB-Buf can induce cell apoptosis, ferroptosis, and ROS production. MB-Buf can be used for the research of cancer, such as breast cancer^[1].

MB-Buf (5 μM, 660 nm laser irradiation for 0-5 min) 会诱导单线态氧、超氧阴离子和羟基自由基的产生^[1]。
MB-Buf (0.1-1 μM, 6 h pretreatment with 660 nm laser irradiation for 5 min) 对 MCF-7、MDA-MB-231 和 4T1 细胞表现出最强的抑制活性，其 IC₅₀ 值分别为 28.74、36.82 和 64.41 nM^[1]。
MB-Buf (0.1-1 μM, 6 h pretreatment with 660 nm laser irradiation for 5 min) 会诱导 MCF-7 细胞中活性氧的产生和铁死亡^[1]。
MB-Buf (10-100 nM, 6 h pretreatment + 660 nm laser irradiation for 2-5 min) 会降解 MCF-7 细胞中的 GPX4^[1]。
MB-Buf (0.5 μM, 6 h pretreatment with 660 nm laser irradiation for 5 min) 会抑制 MCF-7 细胞的迁移^[1]。
MB-Buf (0.5 μM, 6 h pretreatment with 660 nm laser irradiation for 5 min) 会诱导 MCF-7 细胞的凋亡^[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

MB-Buf (1 mg/kg，静脉注射，每 3 天一次) 联合光照可抑制 4 T1-Luci 异种移植小鼠的肿瘤生长^[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

774.02

C₄₇H₅₅N₃O₅S

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Mo X, et al. Design of photoactivatable methylene blue-bufalin conjugate for GPX4-targeted degradation to induce ferroptosis-like death in breast cancer therapy. Bioorg Chem. 2025 Aug;163:108629.  [Content Brief]