1. Academic Validation
  2. Protein tyrosine phosphatase controls breast cancer invasion through the expression of matrix metalloproteinase-9

Protein tyrosine phosphatase controls breast cancer invasion through the expression of matrix metalloproteinase-9

  • BMB Rep. 2013 Nov;46(11):533-8. doi: 10.5483/bmbrep.2013.46.11.053.
Bo-Mi Hwang 1 Hee Suk Chae Young-Ju Jeong Young-Rae Lee Eun-Mi Noh Hyun Zo Youn Sung Hoo Jung Hong-Nu Yu Eun Yong Chung Jong-Suk Kim
Affiliations

Affiliation

  • 1 Departments of Biochemistry, Chonbuk National University Medical School, Jeonju 560-756, Korea jsukim@chonbuk.ac.kr.
Abstract

The expression of Matrix Metalloproteinases (MMPs) produced by Cancer cells has been associated with the high potential of metastasis in several human carcinomas, including breast Cancer. Several pieces of evidence demonstrate that Protein tyrosine phosphatases (PTP) have functions that promote cell migration and metastasis in breast Cancer. We analyzed whether PTP inhibitor might control breast Cancer invasion through MMP expression. Herein, we investigate the effect of 4-hydroxy-3,3-dimethyl-2H benzo[g]indole-2,5(3H)-dione (BVT948), a novel PTP inhibitor, on 12-O-tetradecanoyl phorbol-13-acetate (TPA)-induced MMP-9 expression and cell invasion in MCF-7 cells. The expression of MMP-9 and cell invasion increased after TPA treatment, whereas TPA-induced MMP-9 expression and cell invasion were decreased by BVT948 pretreatment. Also, BVT948 suppressed NF-κB activation in TPA-treated MCF-7 cells. However, BVT948 didn't block TPA-induced AP-1 activation in MCF-7 cells. Our results suggest that the PTP inhibitor blocks breast Cancer invasion via suppression of the expression of MMP-9.

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