1. Academic Validation
  2. Nano-selenium protects grass carp hepatocytes against 4-tert-butylphenol-induced mitochondrial apoptosis and necroptosis via suppressing ROS-PARP1 axis

Nano-selenium protects grass carp hepatocytes against 4-tert-butylphenol-induced mitochondrial apoptosis and necroptosis via suppressing ROS-PARP1 axis

  • Fish Shellfish Immunol. 2023 Mar 14;135:108682. doi: 10.1016/j.fsi.2023.108682.
Jiawen Cui 1 Minna Qiu 1 Yuhang Liu 1 Yuhao Liu 1 You Tang 2 Xiaohua Teng 3 Shu Li 4
Affiliations

Affiliations

  • 1 College of Animal Science and Technology, Northeast Agricultural University, Harbin, 150030, People's Republic of China.
  • 2 Digital Agriculture Key Discipline of Jilin Province, JiLin Agricultural Science and Technology University, Jilin, 132101, People's Republic of China.
  • 3 College of Animal Science and Technology, Northeast Agricultural University, Harbin, 150030, People's Republic of China. Electronic address: tengxiaohua@neau.edu.cn.
  • 4 College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People's Republic of China. Electronic address: lishu@neau.edu.cn.
Abstract

4-tert-butylphenol (4-tBP) is a monomer widely used in the synthesis of industrial chemicals, and posed a high risk to aquatic Animals. Our study focused on toxic phenotype and mechanism of detoxification in grass carp hepatocytes (L8824) after 4-tBP-treatment. In this experiment, L8824 displayed hallmark phenotypes of Apoptosis and Necroptosis after 4-tBP exposure, as evidenced by changes in cell morphology, increased rates of Apoptosis and necrosis, the loss of MMP, the accumulation of ROS, and changes in associated factors (PARP1, JNK, Bid, Bcl-2, Bax, AIFM1, CytC, Caspase 9, APAF1, Caspase 3, TNF-α, TNFR1, RIPK1, RIPK3, and MLKL). Furthermore, we found that 4-tBP-induced Apoptosis and Necroptosis were reversed by pretreating with N-Acetylcysteine (a ROS scavenger) and 3-Aminobenzamide (a PARP1 Inhibitor), indicating that 4-tBP induced the onset of mitochondrial Apoptosis and Necroptosis in L8824 via activating ROS-PARP1 axis. Nano-selenium (Nano-Se) is a novel form of Se with a noteworthy antioxidant capacity. Here, Nano-Se was found to have preventive, therapeutic, and resistance effects on 4-tBP-induced L8824 Apoptosis and Necroptosis. Nano-Se co-treatment with 4-tBP was an optimal way to alleviate 4-tBP-induced Apoptosis and Necroptosis. We demonstrated for the first time that Nano-Se protected L8824 against 4-tBP-induced mitochondrial Apoptosis and Necroptosis through ROS-PARP1 pathway. This study will provide a new theoretical basis for 4-tBP toxicology researches and aquatic animal protection.

Keywords

Apoptosis; Grass carp; Hepatocytes; Nano-Se; Necroptosis.

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