2. Cell Cycle/DNA Damage Epigenetics
  3. PARP
  4. 3-Aminobenzamide

3-Aminobenzamide  (Synonyms: PARP-IN-1)

目录号: HY-12022 纯度: 99.99%
COA 产品使用指南

摩尔计算器

3-Aminobenzamide (PARP-IN-1) 是一种有效的 PARP 抑制剂，在 CHO 细胞中，对 PARP 的 IC50 值约为 50 nM。

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3-Aminobenzamide Chemical Structure

3-Aminobenzamide Chemical Structure

CAS No. : 3544-24-9

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     可免费申领三个不同产品的试用装。

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规格 价格 是否有货 数量
10 mM * 1 mL in Water ￥500
In-stock
200 mg ￥450
In-stock
500 mg ￥800
In-stock
1 g   询价  
5 g   询价  

* Please select Quantity before adding items.

3-Aminobenzamide 相关抗体

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Top Publications Citing Use of Products

    3-Aminobenzamide purchased from MCE. Usage Cited in: J Cell Biochem. 2019 Apr;120(4):4813-4826.  [Abstract]

    In the 3-Aminobenzamide (3AB) plus TGF-β1 group, the phosphorylation of target substrates of mTOR (S6K1 and 4E-BP) is conformably lower than TGF-β1 treatment, as shown by western blotting.

    3-Aminobenzamide purchased from MCE. Usage Cited in: J Cell Biochem. 2019 Apr;120(4):4813-4826.  [Abstract]

    3-Aminobenzamide (3AB; 10 uM) clearly attenuates the cardiac fibrosis triggered by TGF-β1, as demonstrated by the reduction in protein levels of FN and Col I .

    3-Aminobenzamide purchased from MCE. Usage Cited in: J Cell Biochem. 2019 Apr;120(4):4813-4826.  [Abstract]

    In the 3-Aminobenzamide (3AB) plus TGF-β1 group, the phosphorylation of target substrates of mTOR (ULK1) is conformably lower than TGF-β1 treatment, as shown by western blotting.

    3-Aminobenzamide purchased from MCE. Usage Cited in: J Cell Biochem. 2019 Apr;120(4):4813-4826.  [Abstract]

    The protein expression of PARP1 and the phosphorylation levels of mTOR target substrates, including S6K1, 4E-BP, and ULK1, are partially reversed in the abdominal aortic constriction (AAC) model after treatment with 3AB or Rapamycin.

    3-Aminobenzamide purchased from MCE. Usage Cited in: Mol Cell Endocrinol. 2018 Oct 15;474:137-150.  [Abstract]

    Rat heart tissues, which are injected with Ad-PARP1 or treated with 3AB following AAC surgery, the nuclear fraction are immunoprecipitated with anti-PARP1 antibody and subsequently subjected to immunoblotting analysis. n=3.

    3-Aminobenzamide purchased from MCE. Usage Cited in: Biochim Biophys Acta. 2016 Dec;1863(12):3027-3039.  [Abstract]

    The protein expression of PAR and PARP1, FoxO3 and phosphorylated FoxO3 (T32, S252 and S314), and FoxO3 in the nucleus and cytoplasm is measured byWestern blotting. The results are normalized to those of α-tubulin/Lamin B1 and are presented as the means ± SEM.

    查看 PARP 亚型特异性产品:

    查看所有亚型
    PARP PARP1 PARP2 PARP3 PARP4 TNKS1/PARP5A TNKS2/PARP5B PARP7 PARP10 PARP14 PARP15 PARP12 PARP16

    • 生物活性

    • 实验参考方法

    • 纯度 & 产品资料

    • 参考文献

    生物活性

    3-Aminobenzamide (PARP-IN-1) is a potent inhibitor of PARP with IC50 of appr 50 nM in CHO cells, and acts as a mediator of oxidant-induced myocyte dysfunction during reperfusion.

    IC50 & Target[1]

    PARP

    50 nM (IC50)

    体外研究
    (In Vitro)

    3-Aminobenzamide (PARP-IN-1) (>1 μM) causes more than 95% inhibition of PARP activity without significant cellular toxicity. INO-1001 significantly sensitizes CHO cells by blocking most of the DNA repair occurring between radiation fractions[1]. 3-Aminobenzamide significantly improves endothelial function by enhancing the acetylcholine-induced, endothelium-dependent, nitric oxide mediated vasorelaxation after exposure with 400 μM H2O2[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    3-Aminobenzamide 相关抗体:
    体内研究
    (In Vivo)

    In a db/db (Leprdb/db) mouse model, 3-Aminobenzamide ameliorates diabetes-induced albumin excretion and mesangial expansion, and also decreases diabetes-induced podocyte depletion[3]. 3-Aminobenzamide (1.6 mg/kg via intracerebral injection) prevents NAD+ depletion and improves water maze performance after controlled cortical impact (CCI) in mice[4].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    分子量

    136.15

    Formula

    C7H8N2O
    CAS 号
    性状

    固体

    颜色

    Off-white to light brown

    运输条件

    Room temperature in continental US; may vary elsewhere.
    储存方式
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    溶解性数据
    In Vitro: 

    H2O 中的溶解度 : ≥ 11.11 mg/mL (81.60 mM)

    * "≥" means soluble, but saturation unknown.

    配制储备液
    浓度 溶剂体积 质量 1 mg 5 mg 10 mg
    1 mM 7.3448 mL 36.7242 mL 73.4484 mL
    5 mM 1.4690 mL 7.3448 mL 14.6897 mL
    查看完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
    储备液的保存方式和期限：-80°C, 2 years; -20°C, 1 year。-80°C储存时，请在2年内使用， -20°C储存时，请在1年内使用。

    * 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

    • 摩尔计算器

    • 稀释计算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    质量
    =
    浓度
    ×
    体积
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    浓度 (start)

    C1

    ×
    体积 (start)

    V1

    =
    浓度 (final)

    C2

    ×
    体积 (final)

    V2

    In Vivo:

    以下溶解方案，请直接配置工作液。建议现用现配，在短期内尽快用完。 以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比； 如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶。

    • 方案 一

      请依序添加每种溶剂： PBS

      Solubility: 25 mg/mL (183.62 mM); 澄清溶液; 超声助溶

    动物溶解方案计算器
    请输入动物实验的基本信息：

    给药剂量

    mg/kg

    动物的平均体重

    g

    每只动物的给药体积

    μL

    动物数量

    只
    由于实验过程有损耗，建议您多配一只动物的量
    计算结果
    工作液所需浓度 : mg/mL
    您所需的储备液浓度超过该产品的实测溶解度，如有需要，请与 MCE 中国技术支持联系。
    纯度 & 产品资料

    纯度: 99.99%

    COA (189 KB) HNMR (121 KB) RP-HPLC (260 KB)

    产品使用指南 (1538 KB)
    参考文献
    Kinase Assay
    [1]

    PARP activity is measured with a PARP Activity Assay Kit. This method measures relative PARP activity by determining the level of incorporation of 3H-NAD into trichloroacetic acid (TCA) precipitable material in the presence of sheared genomic DNA, which activates PARP. The reaction mixture is added directly to washed cultures in 12-well culture plates and the reaction is allowed to proceed for 60 minutes at 37°C before the cells are removed mechanically, transferred to a microcentrifuge tube, and precipitated with ice-cold 5% TCA.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Animal Administration
    [3]

    Male db/db (Leprdb/db) mice, together with nondiabetic control db/m mice on C57BLKs/J background, are used. INO-1001 and PJ-34 treatment are initiated at 5 weeks of age. In sterile water that is sweetened with NutraSweet, 4.8 g/L 3-Aminobenzamide and 2.4 g/L PJ-34 is dissolved. Control animals receive sweetened water only. The average inhibitor consumption is 60 mg/kg 3-Aminobenzamide and 30 mg/kg PJ-34.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    参考文献

    3-Aminobenzamide 相关分类

    完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
    储备液的保存方式和期限：-80°C, 2 years; -20°C, 1 year。-80°C储存时，请在2年内使用， -20°C储存时，请在1年内使用。

    可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
    H2O 1 mM 7.3448 mL 36.7242 mL 73.4484 mL 183.6210 mL
    5 mM 1.4690 mL 7.3448 mL 14.6897 mL 36.7242 mL
    10 mM 0.7345 mL 3.6724 mL 7.3448 mL 18.3621 mL
    15 mM 0.4897 mL 2.4483 mL 4.8966 mL 12.2414 mL
    20 mM 0.3672 mL 1.8362 mL 3.6724 mL 9.1811 mL
    25 mM 0.2938 mL 1.4690 mL 2.9379 mL 7.3448 mL
    30 mM 0.2448 mL 1.2241 mL 2.4483 mL 6.1207 mL
    40 mM 0.1836 mL 0.9181 mL 1.8362 mL 4.5905 mL
    50 mM 0.1469 mL 0.7345 mL 1.4690 mL 3.6724 mL
    60 mM 0.1224 mL 0.6121 mL 1.2241 mL 3.0604 mL
    80 mM 0.0918 mL 0.4591 mL 0.9181 mL 2.2953 mL

    * 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    3-Aminobenzamide3544-24-9PARP-IN-1PARPpoly ADP ribose polymeraseInhibitorinhibitorinhibit

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