MQA-P 

MQA-P is a multifunctional near-infrared (NIR) fluorescent probe for simultaneously detecting ONOO^-, viscosity, and polarity within mitochondria. MQA-P exhibits a remarkable turn-on response to ONOO^- (λ_em=645 nm) and is highly sensitive to viscosity/polarity in the NIR channel with λ_em>704 nm. MQA-P exhibits excited-state intramolecular charge transfer (ESICT) feature that is highly polarity-sensitive by engineering N,N-dimethylamino as the electron donor and a quinoline cationic unit as the electron acceptor. MQA-P is used for ferroptosis or cancer diagnosis in vitro and in vivo via dual-channel images^[1].

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. 将 MQA-P 溶解在二甲基亚砜 (DMSO) 中以制备储备液 (1.0 mM)。
2. 用于活细胞中 ONOO^- 的成像。
HeLa 细胞与 MQA-P (5 μM) 孵育 30 分钟作为对照；用 SIN-1 (HY-126849; 100 μM) 预处理 30 分钟，然后用 MQA-P (5 μM) 再孵育 30 分钟。绿色通道 (λ_ex=405 nm，λ_em=550-670 nm)。
3. 活细胞黏度成像。
HeLa 细胞与 MQA-P (5 μM) 孵育 30 分钟作为对照；用莫能菌素 (HY-N4302; 10 μM) 预处理 30 分钟，然后与 MQA-P (5 μM) 孵育 30 分钟。荧光图像使用共焦激光扫描显微镜，红色通道 (λ_ex=561 nm，λ_em=680-750 nm)。
4. 用于铁死亡过程中 ONOO^-、粘度和极性的双通道成像。
HeLa 细胞与 MQA-P (5 μM) 孵育 30 分钟作为对照；用 Erastin (HY-15763; 50 μM) 预处理 30 分钟，然后用 MQA-P (5 μM) 再孵育 30 分钟。荧光图像使用共聚焦激光扫描显微镜，绿色通道 (λ_ex=405nm，λ_em=550-670 nm) 用于 ONOO^-，红色通道 (λ_ex=561 nm，λ_em=680-750 nm) 用于粘度和极性^[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. 对于组织切片成像，从小鼠身上分离出正常器官 (包括心脏、肝脏、脾脏、肺和肾脏) 和肿瘤，然后分别切成 5 μm 厚的切片。
2. 这些切片用 MQA-P (20 μM) 孵育30分钟，然后用 PBS (pH 7.4) 洗涤 3 次，使用共聚焦激光扫描显微镜进行活体成像，绿色通道 (λ_ex=405 nm, λ_em=550-670 nm) 用于 ONOO^-，红色通道 (λ_ex=561 nm, λ_em=680-750 nm) 用于粘度和极性^[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

687.60

C₄₀H₃₆BrN₂O₂P

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Li Fan, et al. Multifunctional Fluorescent Probe for Simultaneous Detection of ONOO-, Viscosity, and Polarity and Its Application in Ferroptosis and Cancer Models. Anal Chem. 2023 Apr 4;95(13):5780-5787.  [Content Brief]