o-Pah 

o-Pah is a BODIPY derivative with an -NH₂ and -OH substituted meso-Ph group. o-Pah exhibits metal-induced J-aggregation in the presence of Cu²⁺ and a specific fluorescence enhancement for Hg²⁺ (Ex/Em=483/(495-600) nM)^[1].

o-Pah (20 μM) shows changed colour upon addition of 100 μM different metal ions and the further addition of 20 μM of Cu²⁺ in DMSO/HEPES buffer solution^[1].
Procedures of metal ion sensing Stock solutions of the metal ions (100 mM and 10 mM) were prepared in deionised water^[1].

1. A stock solution of o-Pah (0.5 mM) is prepared in DMSO.
2. The solution of o-Pah is then diluted to 5 μM with HEPES buffer solution (50 mM KNO3, 50 mM HEPRS, pH 7.2).
3. During titration experiments, 2 mL solutions of o-Pah (5 μM, 0.5 μM) are placed in a 1 cm quartz optical cell and Cu²⁺, Hg²⁺ stock solutions are added gradually with a micropipette.
4. In selectivity experiments, the test samples are prepared by adding appropriate amounts of the metal ion stock solution into the 2 mL solution of o-Pah (5 μM).
6. During fluorescence measurements, the excitation wavelength is 483 nm and emission spectra are collected between 495-600 nm.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

355.19

C₁₉H₂₀BF₂N₃O

1181844-41-6

560

483

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Lu H, et al. Specific Cu(2+)-induced J-aggregation and Hg(2+)-induced fluorescence enhancement based on BODIPY. Chem Commun (Camb). 2010 May 28;46(20):3565-7.  [Content Brief]