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o-Pah 是一种带有 -NH2 和 -OH 取代的 meso-Ph 基团的 BODIPY 衍生物。o-Pah 在 Cu2+ 存在下,表现为金属诱导的 J 聚集和对 Hg2+ 特定荧光增强 (Ex/Em=483/(495-600) nM)。

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o-Pah Chemical Structure

o-Pah Chemical Structure

CAS No. : 1181844-41-6

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  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

o-Pah is a BODIPY derivative with an -NH2 and -OH substituted meso-Ph group. o-Pah exhibits metal-induced J-aggregation in the presence of Cu2+ and a specific fluorescence enhancement for Hg2+ (Ex/Em=483/(495-600) nM)[1].

体外研究
(In Vitro)

o-Pah (20 μM) shows changed colour upon addition of 100 μM different metal ions and the further addition of 20 μM of Cu2+ in DMSO/HEPES buffer solution[1].
Procedures of metal ion sensing Stock solutions of the metal ions (100 mM and 10 mM) were prepared in deionised water[1].

1. A stock solution of o-Pah (0.5 mM) is prepared in DMSO.
2. The solution of o-Pah is then diluted to 5 μM with HEPES buffer solution (50 mM KNO3, 50 mM HEPRS, pH 7.2).
3. During titration experiments, 2 mL solutions of o-Pah (5 μM, 0.5 μM) are placed in a 1 cm quartz optical cell and Cu2+, Hg2+ stock solutions are added gradually with a micropipette.
4. In selectivity experiments, the test samples are prepared by adding appropriate amounts of the metal ion stock solution into the 2 mL solution of o-Pah (5 μM).
6. During fluorescence measurements, the excitation wavelength is 483 nm and emission spectra are collected between 495-600 nm.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

355.19

Formula

C19H20BF2N3O

CAS 号
Emission (Em)

560

Excitation (Ex)

483

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

纯度 & 产品资料
参考文献
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产品名称:
o-Pah
目录号:
HY-D1549
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