CD79 蛋白, Human (HEK293, His)

The CD79A protein is critical for initiating the B cell antigen receptor complex (BCR) signaling cascade upon antigen binding. It promotes internalization, transport to late endosomes, and antigen presentation of BCR complexes. CD79 Protein, Human (HEK293, His) is the recombinant human-derived CD79, expressed by HEK293 , with C-6*His labeled tag. ,

CD79A 蛋白对于响应抗原与 B 细胞抗原受体复合物 (BCR) 结合而启动信号转导级联至关重要。它在内化 BCR 复合物、将其运输到晚期内体以及促进抗原呈递方面发挥着至关重要的作用。CD79A 对于 BCR 的表面表达以及原 B 细胞和前 B 细胞的有效分化也是必需的。它通过与 BLNK 结合并使其接近 SYK，从而促进 SYK（一种关键信号分子）的自身磷酸化和激活，从而使 BLNK 磷酸化。CD79A 还与某些 Src 家族酪氨酸激酶（例如 FYN 和 LYN）相互作用，从而增加其活性。值得注意的是，它在未成熟 B 细胞发育过程中抑制 BCR 信号传导。CD79A 与 CD79B 形成二硫键连接的异二聚体，它们一起构成 B 细胞抗原受体复合物，其中 α/β 链异二聚体与由两条重链组成的抗原特异性膜结合表面免疫球蛋白非共价结合，两条轻链。CD79A 通过其磷酸化 ITAM 结构域与 SYK 相互作用，促进 SYK 自身磷酸化和激活。此外，当 Tyr-210 上磷酸化时，CD79A 与 BLNK/SLP65 的 SH2 结构域相互作用，使 BLNK 与 SYK 接近，并允许 SYK 磷酸化 BLNK，这对于 BCR 运输到晚期内体是必需的。

Immobilized Human CD79 at 1 μg/mL (100 μL/well) can bind Anti-CD79B antibody. The ED₅₀ for this effect is 21.28 ng/mL.

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  Immobilized Human CD79 at 1 μg/mL (100 μL/well) can bind Anti- CD79B antibody. The ED₅₀ for this effect is 21.28 ng/mL.

Human

HEK293

C-6*His

P11912-1 (L33-R143)&P40259-1 (A29-D159)

973 & 974

Approximately 25-55 kDa due to the glycosylation.

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.