Ephrin-A1/EFNA1 蛋白, Human (HEK293, Fc)

The Ephrin-A1/EFNA1 protein is a GPI-binding ligand that interacts with Eph receptors and is critical for migration and adhesion. It recruits VAV2, VAV3, and PI3 kinase p85 subunits through EPHA2 phosphorylation, activating RAC1 GTPase for endothelial cell migration. Ephrin-A1/EFNA1 Protein, Human (HEK293, Fc) is the recombinant human-derived Ephrin-A1/EFNA1 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of Ephrin-A1/EFNA1 Protein, Human (HEK293, Fc) is 164 a.a., with molecular weight of 55-60 kDa.

Ephrin-A1/EFNA1 蛋白是一种细胞表面 GPI 结合配体，可与 Eph 受体相互作用，Eph 受体是受体酪氨酸激酶家族，对于神经元、血管和上皮发育过程中的迁移、排斥和粘附至关重要。它与相邻细胞上的 Eph 受体混杂结合，启动接触依赖性双向信号传导。Ephrin-A1/EFNA1 蛋白通过磷酸化 EPHA2 招募 VAV2、VAV3 和 PI3 激酶 p85 亚基，导致 RAC1 GTPase 激活和血管内皮细胞迁移和组装，从而在血管生成和肿瘤新生血管形成中发挥重要作用。此外，它通过酪氨酸磷酸化激活和下调 EPHA2，导致其内化和降解，从而在肿瘤细胞中表现出抗癌作用。此外，Ephrin-A1/EFNA1 蛋白通过下调 EPHA2 和 FAK 在神经胶质瘤肿瘤发生中发挥负调节作用。它可以诱导胚胎神经元生长锥的塌陷并调节树突棘形态发生。Ephrin-A1/EFNA1 蛋白以单体和同二聚体形式存在，并与 EPHA2 形成异二聚体。它与多种受体酪氨酸激酶结合，包括 EPHA1、EPHA2、EPHA3、EPHA4、EPHA5、EPHA6 和 EPHA7，但亲和力较低。

Human

HEK293

C-hFc

P20827 (D19-S182)

1942  [NCBI]

DRHTVFWNSSNPKFRNEDYTIHVQLNDYVDIICPHYEDHSVADAAMEQYILYLVEHEEYQLCQPQSKDQVRWQCNRPSAKHGPEKLSEKFQRFTPFTLGKEFKEGHSYYYISKPIHQHEDRCLRLKVTVSGKITHSPQAHDNPQEKRLAADDPEVRVLHSIGHS

55-60 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.