Frizzled-1 蛋白, Mouse (HEK293, Fc)

Frizzled-1 is a Wnt receptor that is primarily activated by WNT7B and to varying degrees by WNT3A, WNT3, WNT1, and WNT2, while resisting activation by other Wnt proteins. Its fundamental role in canonical Wnt/β-catenin signaling involves promiscuous activation, GSK-3 kinase inhibition, nuclear β-catenin accumulation, and Wnt target gene activation. Frizzled-1 Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived Frizzled-1 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of Frizzled-1 Protein, Mouse (HEK293, Fc) is 180 a.a., with molecular weight of 55-70 kDa.

Frizzled-1 是 Wnt 蛋白的一种受体，可显着被 WNT7B 激活，并在不同程度上被 WNT3A、WNT3、WNT1 和 WNT2 激活，同时对 WNT4、WNT5A、WNT5B、WNT6、WNT7A 或 WNT7B 的激活表现出抗性。某些上下文。它对经典 Wnt/β-连环蛋白信号传导途径的参与至关重要，可促进蓬乱蛋白的激活、GSK-3 激酶的抑制、β-连环蛋白的核积累以及随后 Wnt 靶基因的激活。涉及 PKC 和钙通量的替代信号通路的存在增加了复杂性，其与规范通路的整合存在尚未解决的问题。Frizzled-1 可能在组织形态发生和分化组织中的极性信息转导中发挥至关重要的作用，强调了其多方面的贡献。值得注意的是，与 MYOC 和 WNT7B 的相互作用进一步强调了它在多种细胞过程中的复杂参与。

Mouse

HEK293

C-hFc

O70421 (V69-H248)

14362  [NCBI]

VRAQAAGQVSGPGQQAPPPPQPQQSGQQYNGERGISIPDHGYCQPISIPLCTDIAYNQTIMPNLLGHTNQEDAGLEVHQFYPLVKVQCSAELKFFLCSMYAPVCTVLEQALPPCRSLCERARQGCEALMNKFGFQWPDTLKCEKFPVHGAGELCVGQNTSDKGTPTPSLLPEFWTSNPQH

55-70 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.