Frizzled-4/CD344 蛋白, Rat (HEK293, His)

Frizzled-4/CD344 is a Wnt receptor that mainly activates the β-catenin pathway and affects retinal vascularization. As a receptor for Wnt proteins and Norrin protein (NDP), it stimulates LEF/TCF-mediated transcriptional programs. Frizzled-4/CD344 Protein, Rat (HEK293, His) is the recombinant rat-derived Frizzled-4/CD344 protein, expressed by HEK293 , with C-His labeled tag. The total length of Frizzled-4/CD344 Protein, Rat (HEK293, His) is 144 a.a., with molecular weight of ~23-30 kDa.

Frizzled-4/CD344 是 Wnt 蛋白的受体，主要参与 β-连环蛋白经典信号传导通路，启动涉及蓬乱蛋白、GSK-3 激酶抑制、β-连环蛋白 (CTNNB1) 核积累和 Wnt 靶点激活的级联反应基因。它在视网膜血管形成中的关键作用得到强调，因为它作为 Wnt 蛋白和诺里蛋白 (NDP) 的受体，有助于 β-连环蛋白 (CTNNB1) 的积累和刺激 LEF/TCF 介导的转录程序。Frizzled-4/CD344 可以通过 Wnt 蛋白结合以及通过诺里蛋白 (NDP) 的不依赖于 Wnt 的信号传导来激活。此外，在一些家庭成员中观察到涉及 PKC 和钙通量的第二条途径，可能与规范途径整合。其影响延伸至组织形态发生和极性信息的细胞间传输。Frizzled-4/CD344 与 MAGI3、NDP 的相互作用以及参与与 TSPAN12 和 Norrin (NDP) 的复合物强调了其多方面的作用。TSKU 的竞争性结合以及与磷脂酰肌醇蛋白聚糖 GPC3 的相互作用进一步增加了其在复杂信号网络中的调节功能的细微差别。

Measured by its binding ability in a functional ELISA. Immobilized Human Frizzled-4, at 0.1μg/mL (100 μL/well) can bind Wnt-5a.The ED₅₀ for this effect is 32.28 ng/mL.

Rat

HEK293

C-His

Q9QZH0/NP_072145.1 (F38-E181)

64558  [NCBI]

FGDEEERRCDPIRIAMCQNLGYNVTKMPNLVGHELQTDAELQLTTFTPLIQYGCSSQLQFFLCSVYVPMCTEKINIPIGPCGGMCLSVKRRCEPVLKEFGFAWPDSLNCSKFPPQNDHNHMCMEGPGDEEVPLPHKTPIQPGEE

Approximately 23-30 kDa due to the glycosylation

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.