1. 重组蛋白
  2. Receptor Proteins
  3. G-Protein-Coupled Receptors (GPCRs)
  4. Frizzled
  5. Frizzled-8
  6. Frizzled-8 蛋白, Human (HEK293, His)

Frizzled-8 蛋白, Human (HEK293, His)

目录号: HY-P70915
COA 产品使用指南

Frizzled-8 是一种 Wnt 受体,通过复杂的 Wnt-Fzd-LRP5-LRP6 复合物关键协调 β-连环蛋白信号传导。它通过经典的 Wnt/β-catenin 通路发挥作用,激活乱序蛋白,抑制 GSK-3 激酶,诱导核内 β-catenin 积累,并激活 Wnt 靶基因。Frizzled-8 蛋白, Human (HEK293, His) 是重组的 Frizzled-8 蛋白,由 HEK293 表达,带有 C-6*His 标签。Frizzled-8 蛋白, Human (HEK293, His) 全长 145 个氨基酸,分子量为 22-32 kDa。

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规格 价格 是否有货 数量
10 μg ¥1350 In-stock
50 μg ¥3800 In-stock
100 μg   询价  

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描述

Frizzled-8 is a Wnt receptor that critically coordinates β-catenin signaling through the complex Wnt-Fzd-LRP5-LRP6 complex. It acts through the canonical Wnt/β-catenin pathway, activating scrambled proteins, inhibiting GSK-3 kinase, inducing nuclear β-catenin accumulation, and activating Wnt target genes. Frizzled-8 Protein, Human (HEK293, His) is the recombinant human-derived Frizzled-8 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Frizzled-8 Protein, Human (HEK293, His) is 145 a.a., with molecular weight of 22-32 kDa.

研究背景

Frizzled-8 作为 Wnt 蛋白的受体,在复杂的 Wnt-Fzd-LRP5-LRP6 复合物中发挥着关键作用,通过诱导受体-配体复合物聚集成核糖体大小的信号体来协调 β-连环蛋白信号传导。它主要通过经典的 Wnt/β-连环蛋白信号通路发挥作用,促进杂乱蛋白的激活,抑制 GSK-3 激酶,促进 β-连环蛋白的核积累,并激活 Wnt 靶基因。在一些家族成员中观察到涉及 PKC 和钙通量的额外信号通路,尽管其与经典通路的整合程度仍不清楚。Frizzled-8 可能有助于在组织形态发生和分化组织中转导极性信息。作为 Wnt 蛋白(如 WNT1)的 RYK 共受体,它积极参与 Wnt 信号复合物的形成,与 WNT 蛋白、FZD 蛋白和 LRP5 或 LRP6 结合。与 GPOC、RSPO1、RSPO3 和磷脂酰肌醇蛋白聚糖 GPC3 的相互作用进一步强调了其在多种细胞过程中的复杂参与。

种属

Human

表达系统

HEK293

标签

C-6*His

蛋白编号

Q9H461 (A28-P172)

基因 ID
蛋白结构
N-term
Frizzled-8 (A28-P172)
Accession # Q9H461
6*His
C-term
同用名
frizzled 8; frizzled family receptor 8; Frizzled8; Frizzled-8; FZ-8; FZD8; hFZ8
氨基酸序列

ASAKELACQEITVPLCKGIGYNYTYMPNQFNHDTQDEAGLEVHQFWPLVEIQCSPDLKFFLCSMYTPICLEDYKKPLPPCRSVCERAKAGCAPLMRQYGFAWPDRMRCDRLPEQGNPDTLCMDYNRTDLTTAAPSPPRRLPPPPP

分子量

22-32 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US;may vary elsewhere.

文件资料

Frizzled-8 Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Frizzled-8 Protein, Human (HEK293, His)
目录号:
HY-P70915
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