IFN-beta 蛋白, Human (CHO)

IFN-β (interferon-β) is a key type I interferon cytokine that coordinates the innate immune response to infection, tumors, and inflammation. IFN-β binds to high-affinity (IFNAR2) and low-affinity (IFNAR1) heterodimeric receptors, activates Jak-STAT signaling, and modulates interferon-regulated genes. IFN-beta Protein, Human (CHO) is the recombinant human-derived IFN-beta protein, expressed by CHO , with tag free. The total length of IFN-beta Protein, Human (CHO) is 166 a.a., with molecular weight of 20-23 kDa.

IFN-β（干扰素-β）是一种关键的 I 型干扰素细胞因子，在协调针对各种挑战（包括感染、肿瘤发展和炎症刺激）的先天免疫反应中发挥着关键作用。IFN-β 通过高亲和力 (IFNAR2) 和低亲和力 (IFNAR1) 异二聚体受体发挥作用，触发经典的 Jak-STAT 信号通路，导致干扰素调节基因的转录激活或抑制。这些基因反过来编码对干扰素反应至关重要的效应子，包括抗病毒蛋白、细胞增殖和分化的调节因子以及免疫调节蛋白。虽然主要通过 IFNAR1-IFNAR2 异二聚体受体发出信号，但 IFN-β 还通过单独与 IFNAR1 一起发挥作用并独立于 Jak-STAT 途径发挥作用，从而表现出多功能性。其多方面作用涵盖抗病毒和抗菌活性、调节 B 细胞发育、骨髓生成和脂多糖诱导的肿瘤坏死因子的产生。在神经元稳态领域，IFN-β作为守护者出现，通过促进神经元自噬和α-突触核蛋白清除来调节多巴胺更新并保护多巴胺能神经元，从而避免多巴胺能神经元损失。值得注意的是，IFN-β 的效力超过了干扰素-α (IFN-α)，特别是在诱导细胞凋亡和抗增殖途径方面，这对控制肿瘤细胞生长至关重要。IFN-β 作为单体存在，在多种生理反应和免疫调节中发挥着多才多艺和强大的作用。

Measured in antiviral assays using WISH cells infected with vesicular stomatitis virus and the ED₅₀ is 1-20 pg/mL.

Human

CHO

Tag Free

P01574 (M22-N187)

3456  [NCBI]

MSYNLLGFLQRSSNFQCQKLLWQLNGRLEYCLKDRMNFDIPEEIKQLQQFQKEDAALTIYEMLQNIFAIFRQDSSSTGWNETIVENLLANVYHQINHLKTVLEEKLEKEDFTRGKLMSSLHLKRYYGRILHYLKAKEYSHCAWTIVRVEILRNFYFINRLTGYLRN

20-23 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 10 mM NaAC, 40 mM Arg, 0.12mg/ml Met, 146mg/ml Sucrose, 0.1% PF-68, pH 4.5. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.