1. 重组蛋白
  2. Cytokines and Growth Factors
  3. Interferon & Receptors
  4. IFN-beta
  5. IFN-beta 蛋白, Mouse (HEK293)

IFN-beta 蛋白, Mouse (HEK293)

目录号: HY-P73130
COA 产品使用指南 技术支持

IFN-beta 是一种分泌性细胞因子,由细胞在病毒感染或核酸刺激下产生。IFN-beta 与细胞表面异二聚体受体 (IFNAR1/IFNAR2) 结合,激活 JAK-STAT 信号通路,诱导干扰素刺激基因 (ISG) (如 PKR、2'5'-OAS) 的表达。IFN-beta 能够抑制病毒增殖,调节免疫反应 (如抑制Th1细胞极化、减少促炎细胞因子分泌),抑制血管平滑肌细胞和肿瘤细胞的增殖。IFN-beta 具有抗动脉粥样硬化和血管重塑作用。IFN-beta 蛋白, Mouse (HEK293) 是重组的 IFN-β 蛋白,由 HEK293 表达,不带标签。

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

  • 参考文献

描述

IFN-beta is a secretory cytokine produced by cells under viral infection or nucleic acid stimulation. IFNβ binds to cell surface heterodimeric receptors (IFNAR1/IFNAR2), activates the JAK-STAT signaling pathway, and induces the expression of interferon-stimulated genes (ISGs) (such as PKR, 2'5'-OAS). IFN-beta inhibits viral proliferation, regulates immune responses (such as inhibiting Th1 cell polarization, reducing pro-inflammatory cytokine secretion), inhibits the proliferation of vascular smooth muscle cells and tumor cells. IFN-beta has anti-atherosclerotic and vascular remodeling effects. Mouse (HEK293) is the recombinant mouse-derived IFN-beta protein, expressed by HEK293 , with tag free.

研究背景

IFN-beta 属于 I 型干扰素家族[2][3][7]IFN-beta 是细胞在病毒感染或核酸刺激下产生的分泌型细胞因子[2][5][7]IFN-beta 与细胞表面异二聚体受体 (IFNAR1/IFNAR2) 结合,激活 JAK-STAT 信号通路,诱导干扰素刺激基因 (ISGs) 表达 (如 PKR、2'5'-OAS)[2][3][4][7]IFN-beta 抑制病毒增殖、调节免疫应答 (如抑制 Th1 细胞极化、减少促炎细胞因子分泌)、抑制血管平滑肌细胞和肿瘤细胞增殖,具有抗动脉粥样硬化和血管重塑作用[2][5][6][7]IFN-beta 在脾脏、肺脏、肾脏等组织以及血管内皮细胞、免疫细胞中表达[5][7]IFN-beta Protein, Mouse 与人 IFN-beta 有 50% 的序列同一性[8]

体内研究
(In Vivo)

IFN-beta Protein, Mouse (10000 IU/只小鼠;腹腔注射;每日;5 天) 在 Swiss Webster 小鼠中可增强 DHPG (HY-12598A) 介导的针对 HSV-2 全身感染的保护,降低死亡率[6]
IFN-beta Protein, Mouse (10 MIU/kg;皮下注射;每日;4 周) 可减弱 ApoE-/- 小鼠中血管紧张素 II 诱导的动脉粥样硬化斑块形成,使颈动脉病变面积减少 30%[7]

生物活性

1. Measured in antiviral assays using L929 cells infected with vesicular stomatitisvirus (VSV) and the ED50 is 3-18 pg/mL.
2. Determined by a cytotoxicity assay using human TF-1 cells. The ED50 this effect is ≤0.3901 ng/mL, corresponding to a specific activity is ≥2.563×106 units/mg.
3. Measured by its binding ability in a functional ELISA. Immobilized Recombinant Mouse IFNAR2 at 2 μg/mL (100 μL/well) can bind Biotinylated Recombinant Mouse IFN-beta. The ED50 for this effect is 4.314 ng/mL.

  • Determined by a cytotoxicity assay using human TF-1 cells. The ED50 for this effect is 0.3901 ng/mL, corresponding to a specific activity is 2.563×106 units/mg.
种属

Mouse

表达系统

HEK293

标签

Tag Free

蛋白编号

P01575 (I22-N182)

基因 ID
蛋白结构
N-term
IFN-beta (I22-N182)
Accession # P01575
C-term
蛋白长度

Full Length of Mature Protein

中文名
干扰素-beta 蛋白
同用名
Interferon beta; IFN-beta; IFNB1; IFB; IFNB
氨基酸序列

INYKQLQLQERTNIRKCQELLEQLNGKINLTYRADFKIPMEMTEKMQKSYTAFAIQEMLQNVFLVFRNNFSSTGWNETIVVRLLDELHQQTVFLKTVLEEKQEERLTWEMSSTALHLKSYYWRVQRYLKLMKYNSYAWMVVRAEIFRNFLIIRRLTRNFQN

分子量

Approximately 24-33 kDa due to the glycosylation.

糖基化修饰
Yes
纯度

Greater than 90% as determined by reducing SDS-PAGE

性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4 or PBS, pH 7.4, 8% trehalose.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献

IFN-beta Protein, Mouse (HEK293) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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IFN-beta Protein, Mouse (HEK293)
目录号:
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