IGF-I/IGF-1 蛋白, Human (70a.a)

IGF-I/IGF-1 Protein, Human (70a.a) is a mitogenic cytokine, binds to IGF type 1 receptor, and modulates growth in many tissues, such as nervous tissue, lymphoid tissue, reproductive tissue, smooth muscle, endothelium, and bone; IGF-I/IGF-1 Protein also mediates neuroprotective mechanism.

胰岛素样生长因子（IGF）系统已被证明可以调节许多组织的生长，例如神经组织、淋巴组织、生殖组织、平滑肌、内皮和骨骼。胰岛素样生长因子-1 (IGF-I) 由成骨细胞产生，其促有丝分裂作用是通过其与 IGF 质膜受体的结合介导的。与 IGF-I 和 IGF-II 结合的 IGF 1 型受体被认为是参与介导大多数细胞类型（包括成骨细胞）中这些生长因子的作用的主要受体^[1]。胰岛素样生长因子-1 (IGF-1) 是一种能够介导神经保护和神经可塑性机制的神经营养因子。IGF-1 的靶向过度表达可增强脑损伤小鼠海马新生神经元的生成^[2]。

1.ED₅₀ < 5 ng/mL, measured by a cell proliferation assay using FDC-P1 cells, corresponding to a specific activity of > 2.0×10⁵ units/mg.
2. Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. The ED₅₀ for this effect is ≤100 ng/mL.

• 
  ED₅₀ < 5 ng/mL, measured by a cell proliferation assay using FDC-P1 cells, corresponding to a specific activity of > 2.0 × 10⁵ units/mg.

Human

E. coli

Tag Free

P05019 (G49-A118)

3479  [NCBI]

GPETLCGAELVDALQFVCGDRGFYFNKPTGYGSSSRRAPQTGIVDECCFRSCDLRRLEMYCAPLKPAKSA

7-10 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4 or 20 mM NaAc-HAc, pH 4.5.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Richman C, et al. Recombinant human insulin-like growth factor-binding protein-5 stimulates bone formation parameters in vitro and in vivo. Endocrinology. 1999 Oct;140(10):4699-705.  [Content Brief]

  [2]. Carlson SW, et al. Central Infusion of Insulin-Like Growth Factor-1 Increases Hippocampal Neurogenesis and Improves Neurobehavioral Function after Traumatic Brain Injury. J Neurotrauma. 2018 Jul 1;35(13):1467-1480.  [Content Brief]