LIF 蛋白, Human (HEK293)

LIF protein induces terminal differentiation in leukemic cells, promoting hematopoietic and neuronal cell differentiation, and stimulating acute-phase protein synthesis in hepatocytes. Its diverse activities highlight its role in influencing cellular differentiation across various cell types and physiological contexts. LIF Protein, Human (HEK293) is the recombinant human-derived LIF protein, expressed by HEK293 , with tag free. The total length of LIF Protein, Human (HEK293) is 180 a.a., with molecular weight of 34-55 kDa.

LIF 蛋白具有诱导白血病细胞终末分化的能力。其多种活性包括诱导正常细胞和髓性白血病细胞的造血分化、促进神经元细胞分化以及刺激肝细胞中的急性期蛋白质合成。这些多方面的活动强调了 LIF 在影响各种细胞类型和生理环境的细胞分化中的作用。

1. Measured by its ability to inhibit the proliferation of M1 mouse myeloid leukemia cells and the ED₅₀ is typically 0.2-0.8 ng/mL.
2. Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED₅₀ this effect is ≤0.004637 ng/mL, corresponding to a specific activity is ≥2.157×10⁸ units/mg.
3.Immobilized Human LIF at 2 μg/mL (100 μl/Well) on the plate. Dose response curve for Human LIF R, hFc Tag with the EC₅₀ of ≤0.12 μg/mL determined by ELISA.

• 
  Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED₅₀ for this effect is 0.004534 ng/mL, corresponding to a specific activity is 2.206×10⁸ units/mg.

Human

HEK293

Tag Free

P15018-1 (S23-F202)

3976  [NCBI]

SPLPITPVNATCAIRHPCHNNLMNQIRSQLAQLNGSANALFILYYTAQGEPFPNNLDKLCGPNVTDFPPFHANGTEKAKLVELYRIVVYLGTSLGNITRDQKILNPSALSLHSKLNATADILRGLLSNVLCRLCSKYHVGHVDVTYGPDTSGKDVFQKKKLGCQLLGKYKQIIAVLAQAF

Approximately 24-55 kDa due to the glycosylation

Greater than 95% as determined by reducing SDS-PAGE or Tris-Bis PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM Tris, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.