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  5. LIF 蛋白, Mouse

LIF 蛋白, Mouse

目录号: HY-P7084
COA 产品使用指南

LIF 蛋白, Mouse是参与各种神经和炎症过程的淋巴因子,例如急性期反应,组织损伤和感染。

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规格 价格 是否有货 数量
Free Sample   Apply now  
2 μg ¥350 In-stock
10 μg ¥890 In-stock
50 μg ¥2500 In-stock
100 μg ¥4250 In-stock
> 100 μg   询价  

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Top Publications Citing Use of Products
  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

  • 参考文献

描述

LIF Protein, Mouse is a lymphoid factor involved in various neural and inflammatory processes such as the acute-phase reaction, tissue damage, and infection.

研究背景

白血病抑制因子 (LIF) 已被证明具有多种显着的作用。它从骨组织中释放钙,是阻止正常胚胎干细胞自发分化的分化抑制因子[1]。白血病抑制因子(LIF)参与对损伤的神经和免疫反应。在多种动物和人类炎症条件下,其水平会升高。在某些情况下,例如气管内脂多糖诱导的炎症后,服用白血病抑制因子可以抑制炎症症状。白血病抑制因子还通过下丘脑-垂体-肾上腺轴增加皮质酮水平。外源添加的白血病抑制因子可诱导急性期蛋白表达并刺激促炎细胞因子和单核细胞趋化剂的产生[2]。在造血系统中,LIF诱导某些白血病细胞的分化以及造血干细胞、巨核祖细胞和DA1细胞的增殖。LIF 还具有骨重塑、诱导肝细胞急性期反应、抑制脂肪生成、调节神经分化和抑制肾上皮细胞分化的活性[3]

生物活性

1.The ED50 is <0.01 ng/mL as measured by M1 cells, corresponding to a specific activity of >1.0 × 108 units/mg.
2.Measured by its ability to induce IL-6 secretion by M-NFS-60 mouse cells. The ED50 for this effect is 2.407 ng/mL, corresponding to a specific activity is 4.15×105 U/mg.

  • Measured by its ability to induce IL-6 secretion by M-NFS-60 mouse cells.The ED50 for this effect is 2.407 ng/mL, corresponding to a specific activity is 4.15×105 U/mg.
种属

Mouse

表达系统

E. coli

标签

Tag Free

蛋白编号

P09056 (S24-F203)

基因 ID

16878  [NCBI]

蛋白结构
N-term
LIF (S24-F203)
Accession # P09056
C-term
中文名
重组鼠LIF蛋白
同用名
rMuLIF; Differentiation-stimulating Factor; D factor; MLPLI
氨基酸序列

SPLPITPVNATCAIRHPCHGNLMNQIKNQLAQLNGSANALFISYYTAQGEPFPNNVEKLCAPNMTDFPSFHGNGTEKTKLVELYRMVAYLSASLTNITRDQKVLNPTAVSLQVKLNATIDVMRGLLSNVLCRLCNKYRVGHVDVPPVPDHSDKEAFQRKKLGCQLLGTYKQVISVVVQAFSPLPITPVNATCAIRHPCHGNLMNQIKNQLAQLNGSANALFISYYTAQGEPF

分子量

Approximately 19.9 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末

组分

Lyophilized after extensive dialysis against 50 mM Tris, 150 mM NaCl, pH 8.0 or 20mM PB, 150mM NaCl, pH 7.4,50 mM Tris-HCL, 300 mM NaCL, pH 8.0.

内毒素含量

<1EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
LIF Protein, Mouse
目录号:
HY-P7084
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