1. 重组蛋白
  2. Cytokines and Growth Factors
  3. Neurotrophic Factors
  4. Leukemia Inhibitory Factor
  5. LIF 蛋白, Mouse (HEK293)

LIF 蛋白, Mouse (HEK293)

目录号: HY-P73279
COA 产品使用指南

LIF(白血病抑制因子)促进白血病细胞的终末分化,诱导造血细胞和神经元细胞分化。它还刺激肝细胞中的急性期蛋白质合成。LIF 的多方面作用强调了其在调节造血、神经发生和肝脏反应中的重要性。LIF 蛋白, Mouse (HEK293) 是重组的 LIF 蛋白,由 HEK293 表达,不带标签。LIF 蛋白, Mouse (HEK293) 全长 180 个氨基酸,分子量约为 ~35.6 kDa。

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3.  试用装只面向终端客户

规格 价格 是否有货 数量
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2 μg ¥350 In-stock
10 μg ¥1000 In-stock
50 μg ¥2850 In-stock
100 μg ¥4800 In-stock
500 μg ¥13500 询价
> 100 μg   询价  

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描述

LIF (Leukemia Inhibitory Factor) prompts terminal differentiation in leukemic cells, inducing hematopoietic and neuronal cell differentiation. It also stimulates acute-phase protein synthesis in hepatocytes. LIF's multifaceted role underscores its significance in regulating hematopoiesis, neurogenesis, and hepatic responses. LIF Protein, Mouse (HEK293) is the recombinant mouse-derived LIF protein, expressed by HEK293 , with tag free. The total length of LIF Protein, Mouse (HEK293) is 180 a.a., with molecular weight of ~35.6 kDa.

研究背景

LIF(白血病抑制因子)具有促进白血病细胞终末分化的能力,具有多种活性。值得注意的是,它诱导正常细胞和髓性白血病细胞的造血分化,促进神经元细胞分化,并刺激肝细胞中急性期蛋白的合成。这种多方面的作用强调了 LIF 在协调各种细胞过程中的重要性,有助于调节造血、神经发生和肝脏反应。

生物活性

1. Measured by its ability to inhibit the proliferation of M1 mouse myeloid leukemia cells.The ED50 for this effect is typically 0.1-0.5 ng/mL.
2. Measured in a cell proliferation assay using M-NFS-60 cells. The ED50 this effect is 69.78 ng/mL, corresponding to a specific activity is 1.4331×104 units/mg.

  • Measured in a cell proliferation assay using M-NFS-60 cells. The ED50 this effect is 69.78 ng/mL, corresponding to a specific activity is 1.4331×104 units/mg.
种属

Mouse

表达系统

HEK293

标签

Tag Free

蛋白编号

P09056/NP_032527.1 (S24-F203)

基因 ID

16878  [NCBI]

蛋白结构
N-term
LIF (S24-F203)
Accession # P09056
C-term
同用名
LIF; Leukemia inhibitory factor; HILDA; D factor; MLPLI
氨基酸序列

SPLPITPVNATCAIRHPCHGNLMNQIKNQLAQLNGSANALFISYYTAQGEPFPNNVEKLCAPNMTDFPSFHGNGTEKTKLVELYRMVAYLSASLTNITRDQKVLNPTAVSLQVKLNATIDVMRGLLSNVLCRLCNKYRVGHVDVPPVPDHSDKEAFQRKKLGCQLLGTYKQVISVVVQAF

分子量

Approximately 35.6 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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