LILRA4/ILT-7/CD85g 蛋白, Human (HEK293, His)

The LILRA4/ILT-7/CD85g protein is a coreceptor that critically limits the innate immune response during viral infection through FCER1G signaling. It negatively regulates TLR7 and TLR9 signaling and reduces IFNA1, IFNA2, IFNA4, IFNB1, and TNF production in dendritic cells exposed to influenza virus or CpG dinucleotides. LILRA4/ILT-7/CD85g Protein, Human (HEK293, His) is the recombinant human-derived LILRA4/ILT-7/CD85g protein, expressed by HEK293 , with C-His labeled tag. The total length of LILRA4/ILT-7/CD85g Protein, Human (HEK293, His) is 423 a.a., with molecular weight of ~48.1 KDa.

LILRA4/ILT-7/CD85g 蛋白作为辅助受体，在病毒感染期间限制先天免疫反应中发挥关键作用，信号传导通过 FCER1G 发生。它作为 TLR7 和 TLR9 信号级联的负调节因子，通过其下调暴露于流感病毒或 CpG 二核苷酸的浆细胞样树突细胞中 IFNA1、IFNA2、IFNA4、IFNB1 和 TNF 的产生的能力来证明。此外，LILRA4/ILT-7/CD85g 响应与 HIV-1 感染细胞上的 BST2 的相互作用，负向调节干扰素的产生。该蛋白与 FCER1G 一起激活信号级联，导致 Src 家族和 Syk 激酶磷酸化以及随后细胞内 Ca(2+) 的动员。值得注意的是，LILRA4/ILT-7/CD85g 不会干扰浆细胞样树突状细胞分化为抗原呈递细胞。LILRA4/ILT-7/CD85g 和 FCER1G 之间的相互作用稳定了两种蛋白在细胞膜上的表达，强调了其在针对病毒病原体的先天免疫反应中的关键调节作用。

Measured by its binding ability in a functional ELISA. Immobilized Human LILRA4 at 1 μg/mL (100 μL/well) can bind biotinylated Human ANGPTL7. The ED₅₀ for this effect is 58.08 ng/mL.

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  Measured by its binding ability in a functional ELISA. Immobilized Human LILRA4 at 1 μg/mL (100 μL/well) can bind biotinylated Human ANGPTL7. The ED₅₀ for this effect is 58.08 ng/mL.

Human

HEK293

C-His

P59901-1 (E24-N446)

23547  [NCBI]

ENLLKPILWAEPGPVITWHNPVTIWCQGTLEAQGYRLDKEGNSMSRHILKTLESENKVKLSIPSMMWEHAGRYHCYYQSPAGWSEPSDPLELVVTAYSRPTLSALPSPVVTSGVNVTLRCASRLGLGRFTLIEEGDHRLSWTLNSHQHNHGKFQALFPMGPLTFSNRGTFRCYGYENNTPYVWSEPSDPLQLLVSGVSRKPSLLTLQGPVVTPGENLTLQCGSDVGYIRYTLYKEGADGLPQRPGRQPQAGLSQANFTLSPVSRSYGGQYRCYGAHNVSSEWSAPSDPLDILIAGQISDRPSLSVQPGPTVTSGEKVTLLCQSWDPMFTFLLTKEGAAHPPLRLRSMYGAHKYQAEFPMSPVTSAHAGTYRCYGSRSSNPYLLSHPSEPLELVVSGATETLNPAQKKSDSKTAPHLQDYTVEN

Approximately 60-75 kDa due to the glycosylation

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.