Wnt3a 蛋白, Human (His)

Wnt3a Protein, Human is an activator of the Wnt/β-catenin pathway that inhibits GSK-3β, stabilizes β-catenin and promotes its nuclear translocation to activate FOXM1. Wnt3a Protein is mainly used in studies on neuroprotection in ischemic stroke (such as reducing cerebral infarction volume in MCAO models)^[1][2]. Wnt3a Protein, Human (His) is a recombinant human Wnt3a protein expressed in E. coli with a 6His tag at the N-terminus.

Wnt3a Protein 是一种含糖基化修饰的分泌型糖蛋白，属于 Wnt 家族经典成员，其结构包含 N 端半胱氨酸富集域，可特异性结合 Frizzled 家族受体 (如 Frz1)。Wnt3a Protein 是 Wnt/β-catenin 信号通路的内源性激动剂，其通过与 Frz1 及共受体 LRP6 结合，抑制下游 GSK-3β 磷酸化，解除 β-catenin 降解并促进其核转位，进而激活 FOXM1 等抗凋亡基因转录，同时下调 Cleaved Caspase-3 等凋亡标志物。此外，Wnt3a 可通过激活 PIWIL1a 增强 β-catenin 稳定性，协同调控细胞存活与分化。Wnt3a Protein 的生物学活性还包括促进人脐带间充质干细胞 (hUCMSCs) 向表皮样细胞分化，通过上调 CK19 表达及 β-catenin 信号实现。Wnt3a Protein 还通过其胞外结构域与磷脂酰肌醇蛋白聚糖 GPC3 和 PKD1 相互作用，参与正常胚胎中胚层发育、尾体节形成和发育中的神经管的形态发生，以及在体外介导肠隐窝基部干细胞的自我更新。Wnt3a Protein 主要用于缺血性卒中的神经保护 (如减少 MCAO 模型脑梗死体积、改善神经功能) 及皮肤组织工程中的干细胞定向分化诱导，为卒中治疗及创伤修复提供潜在靶点^[1][2]。

Wnt3a Protein (人源；100 ng/mL；5 天) 可通过激活人脐带间充质干细胞 (hUCMSCs) 中 Wnt/β-catenin 信号通路，提升表皮样细胞标志物 CK19 的 mRNA 及蛋白表达水平，增加 CK19 阳性细胞比例，并上调 β-catenin 表达 ，从而促进 hUCMSCs 向表皮样细胞分化^[1]。

Wnt3a Protein (人源；1.2 μg/kg；单剂量；MCAO 后 1 小时鼻内给药) 在大鼠短暂性 MCAO 模型中，激活 Frz1/PIWIL1a/FOXM1 通路，显著减少 24 和 72 小时的脑梗死体积，改善神经功能评分，并降低神经元凋亡标志物裂解的 Caspase-3 表达，但在永久性MCAO模型中无明显保护作用^[2]。

Human

E. coli

N-6*His

P56704-1 (S19-C351)

89780  [NCBI]

Partial

SYPIWWSLAVGPQYSSLGSQPILCASIPGLVPKQLRFCRNYVEIMPSVAEGIKIGIQECQHQFRGRRWNCTTVHDSLAIFGPVLDKATRESAFVHAIASAGVAFAVTRSCAEGTAAICGCSSRHQGSPGKGWKWGGCSEDIEFGGMVSREFADARENRPDARSAMNRHNNEAGRQAIASHMHLKCKCHGLSGSCEVKTCWWSQPDFRAIGDFLKDKYDSASEMVVEKHRESRGWVETLRPRYTYFKVPTERDLVYYEASPNFCEPNPETGSFGTRDRTCNVSSHGIDGCDLLCCGRGHNARAERRREKCRCVFHWCCYVSCQECTRVYDVHTC

Approximately 40 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0 or PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Sun TJ, et al. Wnt3a promotes human umbilical cord mesenchymal stem cells to differentiate into epidermal-like cells. Eur Rev Med Pharmacol Sci. 2015 Jan;19(1):86-91.  [Content Brief]

  [2]. Matei N, et al. Intranasal wnt3a Attenuates Neuronal Apoptosis through Frz1/PIWIL1a/FOXM1 Pathway in MCAO Rats. J Neurosci. 2018 Jul 25;38(30):6787-6801.  [Content Brief]