2. Apoptosis NF-κB MAPK/ERK Pathway Immunology/Inflammation
  3. RIP kinase NF-κB p38 MAPK TNF Receptor Interleukin Related
  4. RIPK1-IN-32

RIPK1-IN-32 是一种具有抗炎活性的 RIPK 抑制剂。RIPK1-IN-32 能抑制一氧化氮 (NO) 的释放，其 IC50 值为 3.26 μM。RIPK1-IN-32 通过 RIPK1/NF-κB/MAPK 通路，阻止了从而阻止 p65 和 c-fos 的核转位，从而导致 TNF-α 和 IL-6 的表达降低，从而显著减轻与脓毒症相关的急性肝损伤。RIPK1-IN-32 可用于急性肝损伤和脓毒症的研究。

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RIPK1-IN-32

RIPK1-IN-32 Chemical Structure

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RIPK1-IN-32 相关抗体

Top Publications Citing Use of Products

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RIPK1 RIPK2 RIPK3

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NF-κB NF-κB1/p50 RelA/p65 RelB c-Rel

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p38 MAPK p38α p38β MAPK13 p38δ p38γ

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TNFRSF1A/CD120a TNFRSF3/CD18 TNFRSF4 TNFRSF5/CD40 TNFRSF6/Fas/CD95 TNFRSF7/CD27 TNFRSF8/CD30 TNFRSF9/4-1BB/CD137 TNFRSF12A/TWEAK TNFRSF16/NGF Receptor/CD271 TNFRSF18/GITR/CD357

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IL-1 IL-2 IL-3 IL-4 IL-5 IL-6 IL-8 IL-10 IL-12 IL-13 IL-15 IL-17 IL-23 IL-7 IL-33 IL-22

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

RIPK1-IN-32 is a RIPK inhibitor with anti-inflammatory activity. RIPK1-IN-32 inhibits nitric oxide (NO) release with an IC50 of 3.26 μM. RIPK1-IN-32 significantly alleviates acute liver injury associated with sepsis through the RIPK1/NF-κB/MAPK pathway, therefore preventing the nuclear translocation of p65 and c-fos, which results in reduced expression of TNF-α and IL-6. RIPK1-IN-32 can be used for the study of acute liver injury and sepsis[1].

IC50 & Target[1]

NF-κB

 

IL-6

 

RIPK1

 

体外研究
(In Vitro)

RIPK1-IN-32 (Compound 12) (0-25 μM, 45-63 ℃) 显著抑制了 RAW264.7 巨噬细胞中 RIPK1 的热降解[1]。
RIPK1-IN-32 (2.5 μM) 显著减少了 RAW264.7 巨噬细胞中 p65 和 c-fos 的核转移[1]。
RIPK1-IN-32 (0.625-2.5 μM, 8 h) 显著降低了 RAW 264.7 细胞中 IL-6 和 TNF-α 的水平[1]。
RIPK1-IN-32 (0.625-2.5 μM, 8 h) 有效地抑制了 LPS (HY-D1056B3) 诱导的 RIPK1/NF-κB/MAPK 通路的激活[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

RIPK1-IN-32 相关抗体:

ELISA Assay[1]

Cell Line: LPS-stimulated RAW 264.7 macrophages
Concentration: 0.625, 1.25, 2.5 μM
Incubation Time: 8 h, including 1 μg/mL LPS for 6 hours
Result: Significantly diminished the contents of IL-6 and TNF-α.

Western Blot Analysis[1]

Cell Line: LPS-stimulated RAW 264.7 macrophages
Concentration: 0.625, 1.25, 2.5 μM
Incubation Time: 8 h, including 1 μg/mL LPS for 6 hours
Result: Resulted in notable decreases in the levels of RIPK1, p-p65, and p-IκB suggesting an inhibition of NF-κB signaling.
Found to lower the protein levels of p-p38, p-ERK, and p-JNK which aligns with the suppression of MAPK signaling.
体内研究
(In Vivo)

RIPK1-IN-32 (Compound 12) (1.25-5 mg/kg, i.v., 单次给药) 对 LPS 诱导的脓毒症小鼠的肝、肺和肾损伤提供了较大的保护性[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: LPS injection into the tail vein to create a sepsis model established in Male BALB/c mice (18-20 g)[1]
Dosage: 1.25, 2.5 and 5 mg/kg
Administration: Intravenous injection (i.v.), single dose
Result: Markedly alleviated LPS-induced ALI.
Decreased the serum levels of ALT and AST.
Significantly diminished the contents of IL-6 and TNF-α.
Attenuated the damage to lung tissue and markedly lessened the pathological alterations noted in the kidneys subsequent.
Decreased in liver concentrations of RIPK1, p-p65, P-IκB, p-p38, p-ERK, and p-JNK.
分子量

754.05

Formula

C42H59NO7S2
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
纯度 & 产品资料
参考文献

RIPK1-IN-32 相关分类

  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

RIPK1-IN-32RIP kinaseNF-κBp38 MAPKTNF ReceptorInterleukin RelatedReceptor-interacting protein kinasesRIPKNuclear factor-κBNuclear factor-kappaBTumor Necrosis Factor ReceptorTNFRILFusidic acidSepsisAcute liver injuryAnti-inflammatory activityInhibitorinhibitorinhibit

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