2. Stem Cell/Wnt TGF-beta/Smad
  3. TGF-β Receptor TGF-beta/Smad
  4. Sotatercept (mIgG2a)

Sotatercept (mIgG2a)  (Synonyms: RAP-011)

目录号: HY-P99590A 纯度: 99.71%
COA 技术支持

Sotatercept (mIgG2a) (RAP-011)，Sotatercept (ACE-011) (HY-P99590) 的小鼠同源物，是一种可溶性激活素受体 IIA (ActRIIA) 配体陷阱。Sotatercept (mIgG2a) 通过结合并中和激活素 A 和其他 TGF-β 超家族成员 (如 Activin A/B、GDF11 和 BMP9/10)，抑制这些配体与其受体的结合，从而调节细胞增殖和分化。Sotatercept (mIgG2a) 主要抑制 SMAD2/3 信号通路，可用于慢性肾病等多种疾病中。Sotatercept (mIgG2a) 降低促红细胞铁调素 (ERFE) 表达，调节铁代谢，促进红细胞生成。Sotatercept (mIgG2a) 具有促进骨形成 (促合成代谢) 和抑制骨吸收 (抗分解代谢) 的双重作用。

MCE 的所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务

1.  客户无需承担相应的运输费用。

2.  同一机构（单位）同一产品试用装仅限申领一次，同一机构（单位）一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户。

规格 价格 是否有货 数量
1 mg ￥7000
In-stock
5 mg 现货 询价
10 mg   询价  
50 mg   询价  

* Please select Quantity before adding items.

Customer Review

Other Forms of Sotatercept (mIgG2a):

Sotatercept (mIgG2a) 相关抗体

Top Publications Citing Use of Products

查看 TGF-β Receptor 亚型特异性产品:

查看所有亚型
ALK1 ALK2 ALK3 ALK4 ALK5 ALK6 ALK7 TGFβR2 ACVR2A ACVR2B

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Sotatercept (mIgG2a) (RAP-011), the murine homolog of Sotatercept (ACE-011) (HY-P99590), is a soluble activin receptor type IIA (ActRIIA) ligand trap. Sotatercept (mIgG2a) inhibits the binding of activin A and other members of the TGF-β superfamily (such as Activin A/B, GDF11 and BMP9/10) to their receptors by combining and neutralizing them, thereby regulating cell proliferation and differentiation. Sotatercept (mIgG2a) mainly inhibits the SMAD2/3 signaling pathway, and can be used in various diseases such as chronic kidney disease. Sotatercept (mIgG2a) reduces the expression of erythropoietic hepcidin (ERFE), regulates iron metabolism, and promotes red blood cell production. Sotatercept (mIgG2a) has a dual effect of promoting bone formation (anabolic) and inhibiting bone resorption (catabolic)[1][2][3][4].

同型

Mouse IgG2a kappa
推荐同型对照抗体
体外研究
(In Vitro)

Sotatercept (mIgG2a) (0.05 g/L, 1 h-5 d) 能够抑制 SMAD2 的磷酸化以及 ERFE 的表达，促使转录因子 GATA1 进入细胞核，并恢复红细胞标志物的基因表达[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Sotatercept (mIgG2a) 相关抗体:

RT-PCR[1]

Cell Line: K562 cells
Concentration: 0.05 g/L with 50 ng/mL GDF11
Incubation Time: 5 d
Result: Significantly upregulated the erythroid differentiation markers (such as KLF1, ABCB6, etc.).
Upregulated the anti-apoptotic gene BCL2 and downregulated the pro-apoptotic genes BAX/BAD.
Significantly reduced the expression of mRNA ERFE.

Western Blot Analysis[1]

Cell Line: K562 cells
Concentration: 0.05 g/L with 50 ng/mL GDF11
Incubation Time: 0.5, 1, 2 h
Result: Significantly inhibited the phosphorylation of SMAD2 induced by GDF1.
Promoted the nuclear translocation of GATA1, accompanied by an increase in nuclear expression of HSP70 and a decrease in nuclear expression of SMAD4.
Significantly reduced the expression of ERFE protein.
体内研究
(In Vivo)

Sotatercept (mIgG2a) (0.1-10 mg/kg，皮下注射，每周两次，持续 2-6 周) 可有效促进大鼠修复过程中的骨形成[2]。
Sotatercept (mIgG2a) (30 mg/kg，腹腔注射，每日一次，持续 10 天) 在促进 Hamp 转基因小鼠的红细胞生成方面表现出显著效果，同时避免了促红细胞生成素 (HY-P70409) 治疗中常见的铁缺乏问题[3]。
Sotatercept (mIgG2a) (10 mg/kg，皮下注射，每周两次，持续 6 周) 可预防慢性肾脏病 (CKD) 小鼠模型中的血管钙化和肾脏纤维化[4]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Closed femoral fracture surgery established in 8-week male Wistar rats[2]
Dosage: 0.6 mg/kg biw (x 2 doses) + 0.35 mg/kg biw (x 3 doses); 0.18 mg/kg biw (x2 doses) + 0.1 mg/kg biw (x 7 doses) and 10mg/kg biw (x 12 doses)
Administration: Subcutaneous injection (s.c.), twice a week for 2-6 weeks
Result: Significantly increased callus length and bone volume at high-dose RAP-011 (10mg/kg biw), but the quality of the newly formed bone tissue was relatively low, and the improvement in mechanical properties was limited.
Demonstrated certain anabolic effects at low-dose pulsed administration.
No obvious effect at continuous low-dose administration.
Animal Model: Iron-deficiency anemia model established in C57BL/6 mice and in hepcidin antimicrobial peptide over expressing mice (Tg-Hamp mice)[3]
Dosage: 30 mg/kg
Administration: Intraperitoneal injection (i.p.), once daily for 10 days
Result: Significantly increase the hemoglobin concentration in both wild-type and Tg-Hamp mice (better).
Promoted the production of red blood cells while not causing excessive depletion of iron reserves.
Significantly increased the proportion of late erythroid precursor cells (the CD44low population).
Did not change the expression level of Hamp.
Animal Model: CKD that stimulated atherosclerotic calcification model established in high fat fed ldlr−/− mice[4]
Dosage: 10 mg/kg
Administration: Subcutaneous injection (s.c.), twice weekly for 6 weeks
Result: Reduced the calcium content and calcium deposits in the aortal.
Increased the markers of vascular smooth muscle (sm22α, αSMA) and reduced osteogenic markers (Runx2, alkaline phosphatase).
Restored the ActRIIA signal and regulated the Wnt signal.
Inhibited fibrosis mediated by Smad, increased the expression of klotho and downregulated the Wnt/Dkk1 signaling pathway.
应用

ELISA, FACS, Functional assay
偶联物

Unconjugated
复溶方法

The product can be reconstituted/diluted with sterile PBS or saline.
分子量

79.67 kDa

性状

液体

颜色

Colorless to light yellow

运输条件

Shipping with dry ice.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
Biological Activity
  • Immobilized Biotinylated Activin A Protein, Mouse (HY-P73241) can bind Sotatercept (mIgG2a). The EC50 for this effect is 472.2 ng/mL.
纯度 & 产品资料
参考文献

Sotatercept (mIgG2a) 相关分类

  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Sotatercept (mIgG2a)RAP-011RAP011RAP 011TGF-β ReceptorTGF-beta/SmadTransforming growth factor beta receptorsTransforming growth factor betaCongenital dyserythropoietic anemia type IIActivin receptor II ligand trapin vitrodrug treatmentAnemiaIronErythropoietinHepcidin antimicrobial peptideInhibitorinhibitorinhibit

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称：

  

* 需求量：

* 客户姓名：

 

* Email：

* 电话：

 

* 公司或机构名称：

   留言给我们：

Bulk Inquiry

Inquiry Information

产品名称：
Sotatercept (mIgG2a)
目录号：
HY-P99590A
需求量：

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

   产品名称：

  

* Lot #：

* 客户姓名：

 

* Email：

   电话：

 

* 部门：

* 公司或机构名称：

 

   留言给我们：

Request for HNMR Report

We have received your request and will respond to you as soon as possible.

嗨！很高兴为您提供帮助！

尊敬的 MCE 客户您好，
请您选择所在区域，我们将转接对应客服为您服务！

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

热 A B C F G H J L N Q S T X Y Z