Trypsin (MS grade) 

Trypsin MS grade is a serine protease enzyme, and hydrolyzes proteins at the carboxyl side of the Lysine or Arginine. Trypsin MS grade activates PAR2 and PAR4. Trypsin MS grade induces cell-to-cell membrane fusion in PDCoV infection by the interaction of S glycoprotein of PDCoV and pAPN. Trypsin MS grade also promotes cell proliferation and differentiation. Trypsin MS grade can be used in the research of wound healing and neurogenic inflammation^{[1][2][3][4][6]}.

IC50: 37 nM (Tyr1173 site, in NR6wtEGFR cells), 37 nM (Tyr992 site, in NR6wtEGFR cells)^[1]

Trypsin MS 级（5 μg/mL，24 或 48 小时）促进猪三角洲冠状病毒 (PDCoV) 在 LLC-PK 细胞中的复制^[2]。
Trypsin MS 级（10 和 50 ng/mL，12 小时）通过促进 LLC-PK 细胞的膜融合来增强 PDCoV 在 LLC-PK 细胞中的细胞间传播^[2]。
Trypsin MS 级 (0.05%, 3 h) 促进 C6 胶质瘤细胞在无血清和无生长因子培养基中的增殖^[3]。
Trypsin MS 级（20 -150 ng/mL，5 天）增强 PBMC 分化^[4]。

本产品可用于蛋白质序列分析，如蛋白质质谱、测序、肽图谱分析。

使用方法
建议使用 1 mM HCl 溶解或稀释。推荐酶切比例为重组胰蛋白酶：目的蛋白=1：20-1：100，最适 pH 为 7.0-8.0。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Trypsin MS 级（在 50μL 生理盐水中每个位点 100-500 μg，皮内注射）诱导小鼠的抓挠行为^[5]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

9002-07-7

固体

White to off-white

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Bhupendra S.Kaphalia. Chapter 16 - Biomarkers of acute and chronic pancreatitis. Biomarkers in Toxicology. 2014, Pages 279-289.

  [2]. Yue-Lin Yang,et al. Trypsin promotes porcine deltacoronavirus mediating cell-to-cell fusion in a cell type-dependent manner. Emerg Microbes Infect. 2020 Feb 24;9(1):457-468.  [Content Brief]

  [3]. H Amano, et al. Trypsin promotes C6 glioma cell proliferation in serum- and growth factor-free medium. Neurosci Res. 1996 Jul;25(3):203-8.  [Content Brief]

  [4]. Michael J. V. White, et al. Trypsin Potentiates Human Fibrocyte Differentiation. PLoS One. 2013; 8(8): e70795.

  [5]. R Costa, et al. Evidence for the role of neurogenic inflammation components in trypsin-elicited scratching behaviour in mice. Br J Pharmacol. 2008 Jul;154(5):1094-103.  [Content Brief]

  [6]. F Schmidlin, et al. Protease-activated receptors: how proteases signal to cells. Curr Opin Pharmacol. 2001 Dec;1(6):575-82.  [Content Brief]

  [7]. Bhupendra S.Kaphalia, et al. Chapter 16 - Biomarkers of acute and chronic pancreatitis. Biomarkers in Toxicology. 2014, Pages 279-289.