Cetuximab (Synonyms: 西妥昔单抗; C225)

目录号: HY-P9905 纯度: 99.70%: Data Sheet SDS COA
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Cetuximab (C225) 是一种人源 IgG1 单克隆抗体，能够抑制 EGFR，SPR 方法测得 Cetuximab 对 EGFR 的 K_d 值为 0.201 nM；Cetuximab 具有高效的抗肿瘤作用。

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CAS No. : 205923-56-4

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规格	价格	是否有货
1 mg	￥2700	In-stock
5 mg	￥5700	In-stock
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100 mg		询价

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Other Forms of Cetuximab:

Cetuximab (powder) 询价

MCE 顾客使用本产品发表的 45 篇科研文献

IHC

Cell Viability Assay

: Cetuximab purchased from MCE. Usage Cited in: J Clin Invest. 2024 Oct 22;134(24):e182217. [Abstract]; Top: Western blots showing protein expression of MOGAT3 in RKO, RKO EC-R, HT29, and HT29 EC-R cells. Representative blots are shown. MOGAT3KO RKO EC-R and HT29 EC-R, along with RKO EC-R-CTRL and HT29 EC-R-CTRL cell lines, were exposed to 2 μM encorafenib/4 μM cetuximab for 96 hours. Bottom: Relative OD value was assessed to determine cell viability by the CCK-8 assay (n = 3).

: Cetuximab purchased from MCE. Usage Cited in: Theranostics. 2024 Aug 26;14(14):5443-5460. [Abstract]; Cell viability by administrating various concentrations of cetuximab (Cmab) (20-160 μg/mL) and 25 μM HNK in HCT116 and LoVo cells.

: Cetuximab purchased from MCE. Usage Cited in: J Control Release. 2024 Nov:375:643-653. [Abstract]; Cell viability of RKO cells after treated by different concentrations of dabrafenib and cetuximab. The concentration gradient of dabrafenib and cetuximab varied from 0.39 to 50 μg/mL.

: Cetuximab purchased from MCE. Usage Cited in: Nat Commun. 2022 Feb 1;13(1):594. [Abstract]; Cetuximab (1.5 mg/kg; i.p.; single dose). Quantitative analysis of P-EGFR in tumor sections observed by IHC analysis.

: Cetuximab purchased from MCE. Usage Cited in: Nat Commun. 2022 Feb 1;13(1):594. [Abstract]; Cetuximab (1.5 mg/kg; i.p.; single dose). Quantitative analysis of P-EGFR in tumor sections observed by western blot analysis

: Cetuximab purchased from MCE. Usage Cited in: Theranostics. 2020 Apr 6;10(11):5107-5119. [Abstract]; The effect of cetuximab and β-elemene in combination (25 μg/mL) with other cell death inhibitors on the cell viability of KRAS mutant HCT116 and Lovo cells after the treatment for 24 h.

: Cetuximab purchased from MCE. Usage Cited in: Theranostics. 2020 Apr 6;10(11):5107-5119. [Abstract]; The expression of positive regulatory proteins for ferroptosis (HO-1 and transferrin) and the negative regulatory proteins for ferroptosis (GPX4, SLC7A11, FTH1, glutaminase, and SLC40A1) were detected by western blotting after the treatment (β-elemene 125 µg/mL, cetuximab 25 µg/mL) for 24 h.

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生物活性
纯度 & 产品资料
参考文献

生物活性

Cetuximab (C225) is a human IgG1 monoclonal antibody that inhibits epidermal growth factor receptor (EGFR), with a K_d of 0.201 nM for EGFR by SPR. Cetuximab has potent antitumor activity^[1].

同型

Human IgG1 kappa

推荐同型对照抗体

Human IgG1 kappa, Isotype Control

反应种属

Human

IC₅₀ & Target^[1]

EGFR

0.147 nM (Kd, Fixed A431 cells)

RDC Antibody

Soluble EGFR

0.201 nM (Kd)

体外研究
(In Vitro)

Cetuximab (C225) 是一种抑制表皮生长因子受体 (EGFR) 的单克隆抗体，SPR 对可溶性 EGFR 的 K_d 为 0.201 nM。通过 ELISA 检测，Cetuximab 在固定 A431 细胞中对 EGFR 的 K_d 也为 0.147 nM^[1]。
Cetuximab (C225；30 nM) 在处理 8 天后时间依赖性地抑制 SCC-1、SCC-11B、SCC-38 和 SCC-13Y 细胞的增殖。Cetuximab (30 nM) 导致 G₀/G₁ 停滞，诱导细胞凋亡，并减少 Rb、p27^KIP1、Bcl-2 和 Bax 在 SCC-13Y 细胞中的表达。Cetuximab (30 nM) 还可增强放射敏感性并增加 SCC-13Y 细胞中放射诱导的细胞凋亡^[3]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究
(In Vivo)

Cetuximab (1 毫克/注射) 对肿瘤体积有影响，但对 UT-SCC-14 异种移植物的影响更为明显。在 UT-SCC-14 异种移植物中，Cetuximab 可显著降低 EGFR、pEGFR 和 Ki67 的表达。Cetuximab 显著降低 MCT1 和 GLUT1 细胞中的表达，但差异在 UT-SCC-14 异种移植物中更为明显^[2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

基因 ID

1956 [NCBI]

Accession

P00533

应用

ELISA, FACS, Functional assay

偶联物

Unconjugated

复溶方法

The product can be reconstituted/diluted with sterile PBS or saline.

分子量

145.54 kDa

CAS 号

205923-56-4

性状

液体

颜色

Colorless to light yellow

中文名称

西妥昔单抗

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

Format

Human IgG1 kappa

Biological Activity

Cetuximab has a significant ADCC effect on A431 cells in a dose-dependent manner.The EC₅₀ for this effect is 43.50 and 39.11 ng/mL.

纯度 & 产品资料

纯度: 99.70%

选择批次：

Data Sheet (535 KB) SDS (251 KB)

COA (267 KB)

抗体抑制剂使用指南 (1152 KB)

参考文献

[1]. Goldstein NI, et al. Biological efficacy of a chimeric antibody to the epidermal growth factor receptor in a human tumor xenograft model. Clin Cancer Res. 1995 Nov;1(11):1311-8. [Content Brief]

[2]. Gustafsson H, et al. EPR Oximetry of Cetuximab-Treated Head-and-Neck Tumours in a Mouse Model. Cell Biochem Biophys. 2017 Jul 29. [Content Brief]

[3]. Huang SM, et al. Epidermal growth factor receptor blockade with C225 modulates proliferation, apoptosis, and radiosensitivity in squamous cell carcinomas of the head and neck. Cancer Res. 1999 Apr 15;59(8):1935-40. [Content Brief]

Cetuximab 相关分类

摩尔计算器
稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量		浓度		体积		分子量 *
	=		×		×

The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

浓度 _(start)	×	体积 _(start)	=	浓度 _(final)	×	体积 _(final)
	×		=		×
C1		V1		C2		V2

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Cetuximab205923-56-4C225西妥昔单抗C 225C-225Radionuclide-Drug Conjugates (RDCs)EGFREpidermal growth factor receptorErbB-1HER1Inhibitorinhibitorinhibit

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MedChemExpress (MCE) 只为有资质的科研机构、医药企业基于科学研究或药证申报的用途提供医药研发服务，不为任何个人或者非科研性质的、非用于药证申报使用等其他用途提供服务。

站点地图隐私声明

沪ICP备15051369号-4

上海工商

沪公网安备31011502019417

沪(浦)应急管危经许[2021]201709(QFYS)

营业执照（三证合一）

Cetuximab (Synonyms: 西妥昔单抗; C225)

Customer Review

Other Forms of Cetuximab:

MCE 顾客使用本产品发表的 45 篇科研文献