1. 诱导疾病模型产品 Cell Cycle/DNA Damage
  2. 肿瘤模型 DNA Alkylator/Crosslinker
  3. 结肠肿瘤模型
  4. Azoxymethane

Azoxymethane  (Synonyms: 偶氮甲烷; AOM)

目录号: HY-111375 纯度: ≥99.0%
COA 产品使用指南

Azoxymethane 是一种结肠致癌物质,可导致 DNA 加合物的形成。

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Azoxymethane Chemical Structure

Azoxymethane Chemical Structure

CAS No. : 25843-45-2

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5 mg (135 mM * 500 μL in Water) ¥2650
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10 mg (135 mM * 1 mL in Water) ¥5000
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Top Publications Citing Use of Products
  • 生物活性

  • 实验参考方法

  • 纯度 & 产品资料

  • 参考文献

生物活性

Azoxymethane is a colon carcinogen which leads to the formation of DNA adducts.

体外研究
(In Vitro)

Azoxymethane 是一种结肠致癌物,可导致 DNA 加合物的形成。在相同的蛋白质基础上,肝微粒体在 NADPH 依赖性 Azoxymethane 生物活化和 N7-mG 加合物形成方面比 SI 和结肠微粒体活跃得多。肝微粒体对氧化 Azoxymethane 的羟基化活性最高,其次是 SI 和结肠微粒体[1]

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究
(In Vivo)

Azoxymethane 可用于动物建模,构建肿瘤模型。

无论何种菌株,氧化 Azoxymethane 产生的 O6-mG 和 N7-mG 的量在肝脏中最高,其次是近端和远端结肠,具有相似的水平,然后是十二指肠、空肠和回肠。结果表明,Azoxymethane 诱导的 SI 和结肠中的 DNA 加合物形成不依赖于肝 P450 酶的生物激活。无论小鼠品系如何,在盐水处理小鼠的结肠中均未检测到异常隐窝病灶 (ACF);相比之下,在氧化 Azoxymethane 处理的所有三种品系小鼠中均检测到结肠 ACF[1]。氧化 Azoxymethane 处理的无胸腺小鼠的肿瘤发生率比类似处理的 WT 动物低约 11 倍[2]

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial
分子量

74.08

Formula

C2H6N2O

CAS 号
性状

液体

颜色

Colorless to light yellow

中文名称

偶氮甲烷

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Solution, -20°C, 2 years

纯度 & 产品资料

纯度: ≥99.0%

参考文献
Kinase Assay
[1]

The assay for Azoxymethane-induced in vitro DNA adduct formation is performed. Briefly, microsomes (0.5 to 2.0 mg/mL) are incubated with calf thymus DNA (1 mg/mL) and Azoxymethane (200 μM) in a total volume of 1.0 mL. The assay buffer consists of 0.1 M Tris-HCl (pH 7.4), 1 mM EDTA, 20 mM MgCl2, 0.3 M KCl, and 1.5 mM NADPH. Incubations are carried out at 37°C for 60 min in a shaking water bath. An additional 30 nM of NADPH is added after the first 30 min. The reaction is stopped by the addition of 0.5 mL of ice-cold 7.5 M ammonium acetate. DNA is then extracted for tissue homogenates. Control incubations are performed without NADPH[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[1]

Male, 8 to 10 week old, WT-A/J, IECN-A/J, and LCN-A/J mice (8 per group) are treated with either saline or Azoxymethane (7.5 mg/kg BW, s.c.), once weekly for 3 weeks. Mice are sacrificed 6 weeks post-treatment for aberrant crypt foci (ACF) detection. The entire colon is excised. A longitudinal incision is made along the entire length of the colon, which is further cut into two equal-length segments, representing proximal and distal portions of the colon. The segments are dipped in PBS to remove fecal pellets and then kept flat between filter papers in 10% buffered formalin for at least 24 h. Subsequently, the colons are immersed in freshly prepared 0.1% methylene blue for 10 min and rinsed briefly in deionized H2O to remove excess dye. The colon is mounted carefully on a microscope slide with the mucosal surface side up and viewed under a light microscope. The ACF in the entire mucosal surface of the colon are counted blindly and independently by two investigators and recorded[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献
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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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