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  2. DAPI dihydrochloride

DAPI dihydrochloride (Synonyms: 4',6-Diamidino-2-phenylindole dihydrochloride)

目录号: HY-D0814 纯度: >98.0%
产品使用指南

DAPI二盐酸盐(4',6-二脒基-2-苯基吲哚)是结合富含A-T碱基对DNA序列的荧光染色。

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DAPI dihydrochloride Chemical Structure

DAPI dihydrochloride Chemical Structure

CAS No. : 28718-90-3

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     可免费申领三个不同产品的试用装。

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Size Price Stock Quantity
10 mM * 1 mL in DMSO ¥550 In-stock
5 mg ¥500 In-stock
10 mg ¥650 In-stock
25 mg ¥1300 In-stock
50 mg ¥2300 In-stock
100 mg   Get quote  
200 mg   Get quote  

* Please select Quantity before adding items.

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Description

DAPI dihydrochloride (4',6-diamidino-2-phenylindole) is a fluorescent stain by binding in the minor grove of A-T rich sequences of DNA.

In Vitro

DAPI (4',6-diamidino-2-phenylindole) is a DNA-specific probe which forms a fluorescent complex by attaching in the minor grove of A-T rich sequences of DNA. It also forms nonfluorescent intercalative complexes with double-stranded nucleic acids. DAPI can be used as a DNA-specific probe for flow cytometry, chromosome staining, DNA visualization and quantitation in histochemistry and biochemistry[1].

Molecular Weight

350.25

Formula

C₁₆H₁₇Cl₂N₅

CAS No.

28718-90-3

SMILES

N=C(C1=CC2=C(C=C1)C=C(C3=CC=C(C(N)=N)C=C3)N2)N.[H]Cl.[H]Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 75 mg/mL (214.13 mM; Need ultrasonic)

H2O : 5 mg/mL (14.28 mM; Need ultrasonic and warming)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.8551 mL 14.2755 mL 28.5510 mL
5 mM 0.5710 mL 2.8551 mL 5.7102 mL
10 mM 0.2855 mL 1.4276 mL 2.8551 mL
* 请根据产品在不同溶剂中的溶解度,选择合适的溶剂配制储备液,并请注意储备液的保存方式和期限。
In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案,配制前请先配制澄清的储备液,再依次添加助溶剂 (为保证实验结果的可靠性,体内实验的工作
液,建议您现用现配,当天使用;澄清的储备液可以根据储存条件,适当保存;以下溶剂前的百分比是指该溶剂在您配制终溶液中的体积占比):

  • 1.

    请依序添加每种溶剂: 10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.08 mg/mL (5.94 mM); Clear solution

  • 2.

    请依序添加每种溶剂: 10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.08 mg/mL (5.94 mM); Clear solution

  • 3.

    请依序添加每种溶剂: 10% DMSO    90% corn oil

    Solubility: ≥ 2.08 mg/mL (5.94 mM); Clear solution

*以上所有助溶剂都可在 MCE 网站选购。
References

Purity: >98.0%

  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

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产品名称:
DAPI dihydrochloride
目录号:
HY-D0814
需求量: