1. Cytoskeleton Neuronal Signaling Autophagy
  2. Myosin CaMK Autophagy
  3. MLCK inhibitor peptide 18

MLCK inhibitor peptide 18  (Synonyms: MLCK抑制肽18)

目录号: HY-P1029 纯度: 99.87%
COA 产品使用指南

MLCK inhibitor peptide 18 是一种有效的 MLCK 抑制剂,IC50 值为 50 nM,同时较弱抑制 CaM kinase II 的活性。

MCE 的所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务

Custom Peptide Synthesis

MLCK inhibitor peptide 18 Chemical Structure

MLCK inhibitor peptide 18 Chemical Structure

CAS No. : 224579-74-2

1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 价格 是否有货 数量
1 mg ¥500
In-stock
5 mg ¥1750
In-stock
10 mg ¥3200
In-stock
25 mg ¥7200
In-stock
50 mg   询价  
100 mg   询价  

* Please select Quantity before adding items.

Customer Review

注册 MCE会员完成审核
即刻享有 积分商城 300 专属积分

Top Publications Citing Use of Products
  • 生物活性

  • 实验参考方法

  • 纯度 & 产品资料

  • 参考文献

生物活性

MLCK inhibitor peptide 18 is a myosin light chain kinase (MLCK) inhibitor with an IC50 of 50 nM, and inhibits CaM kinase II only at 4000-fold higher concentrations.

IC50 & Target

CaMK II

 

体外研究
(In Vitro)

MLCK inhibitor peptide 18 对 MLCK 的肽底物利用具有选择性作用,不干扰 CaM 的激酶激活,对密切相关的 CaMPKII 没有明显的抑制活性,不抑制 PKA[1]

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

1324.62

Formula

C60H105N23O11

CAS 号
性状

固体

颜色

White to off-white

Sequence

Arg-Lys-Lys-Tyr-Lys-Tyr-Arg-Arg-Lys-NH2

Sequence Shortening

RKKYKYRRK-NH2

中文名称

MLCK抑制肽18

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性数据
In Vitro: 

H2O 中的溶解度 : 100 mg/mL (75.49 mM; 超声助溶)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 0.7549 mL 3.7747 mL 7.5493 mL
5 mM 0.1510 mL 0.7549 mL 1.5099 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

* 备注:如您选择水作为储备液,请稀释至工作液后,再用 0.22 μm 的滤膜过滤除菌后使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

In Vivo:

以下溶解方案,请直接配置工作液。建议现用现配,在短期内尽快用完。 以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比; 如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶。

  • 方案 一

    请依序添加每种溶剂: PBS

    Solubility: 100 mg/mL (75.49 mM); 澄清溶液; 超声助溶

动物溶解方案计算器
请输入动物实验的基本信息:

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

由于实验过程有损耗,建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
该产品水溶性佳,请具体参考实测 水 / PBS / Saline 中的溶解度数据。
您所需的储备液浓度超过该产品的实测溶解度,如有需要,请与 MCE 中国技术支持联系。
纯度 & 产品资料
参考文献
Kinase Assay
[1]

All assays are done in a final volume of 50 μL. Each point is tested in duplicate. The standard assay conditions are 50 mM HEPES, 0.2 mM [γ-32P]ATP (specific activity 200−300 cpm/pmol), 1 mM DTT, 1 mg/mL bovine serum albumin, 5 mM MgCl2, 150 mM KCl, 15 mM NaCl, pH 7.5, with or without inhibitors. Synthetic substrate concentrations, KKRPQRATSNVFAM-NH2 for MLCK, PLRRTLSVAA-NH2 for CaMPKII, and LRRASLG for PKA, are 20 μM. The reactions are initiated with the addition of 5 μL of enzyme (PKA) or enzyme:CaM complex (for MLCK and CaMPKII). Enzyme:CaM complexes are preformed by incubation of enzyme in ice for 1 min in the presence of 1 μM CaM and 5 μM CaCl2 (for MLCK) or 30 μM CaM and 2 mM CaCl2 (for CaMPKII). Assay conditions are 20 min, 25°C for MLCK; 8 min, 25°C for CaMPKII; 6 min, 25°C for PKA. Aliquots of each reaction tube are spotted into phosphocellulose filter paper. Filters are washed in 75 mM phosphoric acid solution, followed by 95% ethanol, dried, and placed into vials containing 10 mL of Ecoscint O. Radioactivity is determined by counting vials in a Beckman 6500 liquid scintillation counter. No quenching corrections are applied. Data are transformed as percent of the maximal enzyme activity, where enzyme activity in the absence of inhibitors is taken as 100%. ICsub>50 values are calculated by a linear regression data analysis on data points corresponding to 15%−85% of maximal activity. This analysis is done using InPlot version 4.0.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献

MLCK inhibitor peptide 18 相关分类

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 0.7549 mL 3.7747 mL 7.5493 mL 18.8733 mL
5 mM 0.1510 mL 0.7549 mL 1.5099 mL 3.7747 mL
10 mM 0.0755 mL 0.3775 mL 0.7549 mL 1.8873 mL
15 mM 0.0503 mL 0.2516 mL 0.5033 mL 1.2582 mL
20 mM 0.0377 mL 0.1887 mL 0.3775 mL 0.9437 mL
25 mM 0.0302 mL 0.1510 mL 0.3020 mL 0.7549 mL
30 mM 0.0252 mL 0.1258 mL 0.2516 mL 0.6291 mL
40 mM 0.0189 mL 0.0944 mL 0.1887 mL 0.4718 mL
50 mM 0.0151 mL 0.0755 mL 0.1510 mL 0.3775 mL
60 mM 0.0126 mL 0.0629 mL 0.1258 mL 0.3146 mL

* 备注:如您选择水作为储备液,请稀释至工作液后,再用 0.22 μm 的滤膜过滤除菌后使用。

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称:

 

* 需求量:

* 客户姓名:

 

* Email:

* 电话:

 

* 公司或机构名称:

   留言给我们:

Bulk Inquiry

Inquiry Information

产品名称:
MLCK inhibitor peptide 18
目录号:
HY-P1029
需求量: