Thymidine (Synonyms: 胸苷; 胸腺嘧啶核苷; DThyd; NSC 21548)

目录号: HY-N1150 一键复制产品信息纯度: 99.94%: Data Sheet SDS COA 产品使用指南
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技术支持

Thymidine （胸苷或胸腺嘧啶核苷）是脱氧核糖核酸的一种特殊前体，被用作细胞同步剂。Thymidine 是一种 DNA 合成抑制因子，它能在 DNA 复制前在 G1/S 边界阻滞细胞。

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规格	价格	是否有货
500 mg	￥400	In-stock
1 g	￥500	In-stock
10 g	￥1500	In-stock
50 g		询价

or 大包装询价

* Please select Quantity before adding items.

Customer Review

Other Forms of Thymidine:

MCE 顾客使用本产品发表的 23 篇科研文献

Flow Cytometry

Cell Imaging/Staining

Cell Proliferation/Viability Assay

: Thymidine purchased from MCE. Usage Cited in: Cell Death Dis. 2025 Sep 29;16(1):673. [Abstract]; Thymidine (2.5 mM, 16 h). Immunoblots of TUFT1 in synchronous HeLa cells. Cyclin B1 served as a marker for M phase. GAPDH served as a loading control.

: Thymidine purchased from MCE. Usage Cited in: Adv Sci (Weinh). 2024 Dec;11(45):e2405441. [Abstract]; IB analysis of WCL derived from T24 cells synchronized at the late G1/S boundary by double-thymidine block, following by releasing back into the cell cycle for the indicated times.

: Thymidine purchased from MCE. Usage Cited in: Nat Struct Mol Biol. 2024 May 20. [Abstract]; Cell viability in day 2 and UMP and dTMP levels were examined in shCtrl and shHES4 A549 cells supplemented with or without 200 μM choline chloride, 50 μM betaine, 2 mM serine, 3 mM sodium pyruvate and 100 μM each of uridine (U) and thymidine (T) for 24 has indicated.

: Thymidine purchased from MCE. Usage Cited in: Adv Sci (Weinh). 2022 Aug;9(22):e2104823. [Abstract]; Thymidine (2 mM, 16 h). KG1A cells were synchronized at the G1/S boundary, released into fresh media, and harvested at the indicated times (T/T release).

: Thymidine purchased from MCE. Usage Cited in: J Hazard Mater. 2022 Jan 5:421:126815.; Chromosome segregation image in live HTR-8 cells in control or Thymidine (2 nM, 16 h) treatment and progesterone treatment (after Thymidine treatment).

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生物活性
纯度 & 产品资料
参考文献

生物活性

Thymidine, a specific precursor of deoxyribonucleic acid, is used as a cell synchronizing agent. Thymidine is a DNA synthesis inhibitor that can arrest cell at G1/S boundary, prior to DNA replication^[1]^[2]^[3].

IC₅₀ & Target^[2]

Microbial Metabolite

DNA Synthesis

Human Endogenous Metabolite

细胞效力
(Cellular Effect)

Cell Line	Type	Value	Description	References
143B	CC₅₀	0.0006 M Compound: Thymidine	In vitro cell cytotoxicity was determined against 143B cell line In vitro cell cytotoxicity was determined against 143B cell line	[PMID: 15027876]
143B	CC₅₀	0.0007 M Compound: Thymidine	In vitro cell cytotoxicity was determined against 143B-LTK cell line In vitro cell cytotoxicity was determined against 143B-LTK cell line	[PMID: 15027876]
CCRF-CEM	CC₅₀	10.6 μM Compound: 1i	Cytotoxicity in human CEM/0 cells assessed as reduction in cell viability incubated for 4 to 5 days Cytotoxicity in human CEM/0 cells assessed as reduction in cell viability incubated for 4 to 5 days	[PMID: 32515595]
CCRF-CEM	EC₅₀	> 40 μM Compound: 3 (d Thd)	Inhibitory effect against HIV-1 replication in CEM cells. Inhibitory effect against HIV-1 replication in CEM cells.	[PMID: 7538589]
CCRF-CEM	EC₅₀	> 40 μM Compound: 3 (d Thd)	Inhibitory effect against HIV-2 replication in CEM /TK-(thymidine kinase deficient) cells. Inhibitory effect against HIV-2 replication in CEM /TK-(thymidine kinase deficient) cells.	[PMID: 7538589]
CCRF-CEM	EC₅₀	> 40 μM Compound: 3 (d Thd)	Inhibitory effect against HIV-2 replication in CEM cells. Inhibitory effect against HIV-2 replication in CEM cells.	[PMID: 7538589]
HL-60	CC₅₀	1760 μM Compound: beta-L-dT	Cytotoxicity against human HL60 cells after 3 days by MTT assay Cytotoxicity against human HL60 cells after 3 days by MTT assay	[PMID: 17404006]
HeLa	IC₅₀	4000 μM Compound: 1	Inhibitory concentration against HeLa cell proliferation after 72 hours of incubation Inhibitory concentration against HeLa cell proliferation after 72 hours of incubation	[PMID: 15582414]
HepG2	CC₅₀	2100 μM Compound: beta-L-dT	Cytotoxicity against human HepG2 cells after 3 days by MTT assay Cytotoxicity against human HepG2 cells after 3 days by MTT assay	[PMID: 17404006]
HepG2 2.2.15	EC₅₀	0.3 μM Compound: beta-L-dT	Antiviral activity against HBV in HepG2.2.15 cells assessed as decrease in extracellular viral DNA level by RT-PCR Antiviral activity against HBV in HepG2.2.15 cells assessed as decrease in extracellular viral DNA level by RT-PCR	[PMID: 17404006]
Huh-7	CC₅₀	> 200 μg/mL Compound: 6	Cytotoxicity against human HuH7 cells Cytotoxicity against human HuH7 cells	[PMID: 20863701]
KBALB-STK	CC₅₀	0.0001 M Compound: Thymidine	In vitro cell cytotoxicity against KBALB-STK cell lines expressed in HSV-1 TK In vitro cell cytotoxicity against KBALB-STK cell lines expressed in HSV-1 TK	[PMID: 12620076]
KBALB-STK	CC₅₀	0.00065 M Compound: Thymidine	In vitro cell cytotoxicity was determined against KBALB-STK cell line In vitro cell cytotoxicity was determined against KBALB-STK cell line	[PMID: 15027876]
Vero	IC₅₀	0.3 nM Compound: Thymidine	Anti-Herpes simplex virus type-1 activity in vero cells using plaque inhibition assay Anti-Herpes simplex virus type-1 activity in vero cells using plaque inhibition assay	[PMID: 10464017]
Vero	IC₅₀	0.4 nM Compound: thymidine	Anti-Herpes simplex virus type-1 activity in vero cells using plaque inhibition assay Anti-Herpes simplex virus type-1 activity in vero cells using plaque inhibition assay	[PMID: 10464017]

体内研究
(In Vivo)

Thymidine (500 mg/kg；腹腔注射；每天两次) 完全逆转甲氨蝶呤和 Tomudex 诱导的 CD4⁺Vβ8⁺ 和 CD8⁺Vβ8⁺ T 细胞缺失^[3]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	8-12 weeks BALB/c mice^[3]
Dosage:	500 mg/kg
Administration:	i.p.; twice a day
Result:	Completely abrogates Methotrexate- and Tomudex-induced deletion of Vβ8⁺ T cells after SEB injection.

Clinical Trial

分子量

242.23

Formula

C₁₀H₁₄N₂O₅

CAS 号

50-89-5

性状

固体

颜色

White to off-white

结构分类

Others

初始来源

微生物

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

溶解性数据

细胞实验：

DMSO 中的溶解度 : 50 mg/mL (206.42 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

H₂O 中的溶解度 : 33.33 mg/mL (137.60 mM; 超声助溶)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	4.1283 mL	20.6415 mL	41.2831 mL
5 mM	0.8257 mL	4.1283 mL	8.2566 mL
10 mM	0.4128 mL	2.0642 mL	4.1283 mL

查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

* 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

摩尔计算器
稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

浓度 _(start)

体积 _(start)

浓度 _(final)

体积 _(final)

动物实验：

以下溶解方案，请直接配制工作液。建议现用现配，在短期内尽快用完。以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶。

方案一

请依序添加每种溶剂： PBS
Solubility: 20 mg/mL (82.57 mM); 澄清溶液; 超声助溶 (<60°C)

动物溶解方案计算器

请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

每只动物的给药体积

μL

动物数量

只

由于实验过程有损耗，建议您多配一只动物的量

计算结果

工作液所需浓度 : mg/mL

该产品水溶性佳，请具体参考实测水 / PBS / Saline 中的溶解度数据。

您所需的储备液浓度超过该产品的实测溶解度，如有需要，请与 MCE 中国技术支持联系。

纯度 & 产品资料

纯度: 99.96%

选择批次：

Data Sheet (626 KB) SDS (393 KB)

COA (289 KB) LCMS (107 KB)

产品使用指南 (1538 KB)

参考文献

[1]. Chen G, et al. Cell Synchronization by Double Thymidine Block. Bio Protoc. 2018 Sep 5;8(17). [Content Brief]

[2]. FIRKET H, et al. Autoradiographic visualization of synthesis of deoxyribonucleic acid in tissue culture with tritium-labelled thymidine. Nature. 1958 Jan 24;181(4604):274-5.FIRKET H, et al. Autoradiographic visualization of synthesis of deoxyribonucleic acid in tissue culture with tritium-labelled thymidine. Nature. 1958 Jan 24;181(4604):274-5. [Content Brief]

[3]. Izeradjene K, et al. Inhibition of thymidine synthesis by folate analogues induces a Fas-Fas ligand-independentdeletion of superantigen-reactive peripheral T cells. Int Immunol. 2001 Jan;13(1):85-93. [Content Brief]

Thymidine 相关分类

完整储备液配制表

可选溶剂	浓度溶剂体积质量	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	4.1283 mL	20.6415 mL	41.2831 mL	103.2077 mL
	5 mM	0.8257 mL	4.1283 mL	8.2566 mL	20.6415 mL
	10 mM	0.4128 mL	2.0642 mL	4.1283 mL	10.3208 mL
	15 mM	0.2752 mL	1.3761 mL	2.7522 mL	6.8805 mL
	20 mM	0.2064 mL	1.0321 mL	2.0642 mL	5.1604 mL
	25 mM	0.1651 mL	0.8257 mL	1.6513 mL	4.1283 mL
	30 mM	0.1376 mL	0.6881 mL	1.3761 mL	3.4403 mL
	40 mM	0.1032 mL	0.5160 mL	1.0321 mL	2.5802 mL
	50 mM	0.0826 mL	0.4128 mL	0.8257 mL	2.0642 mL
	60 mM	0.0688 mL	0.3440 mL	0.6881 mL	1.7201 mL
	80 mM	0.0516 mL	0.2580 mL	0.5160 mL	1.2901 mL
	100 mM	0.0413 mL	0.2064 mL	0.4128 mL	1.0321 mL

* 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Thymidine50-89-5DThyd NSC 21548NSC21548NSC 21548NSC-21548DNA/RNA SynthesisEndogenous MetaboliteOrthopoxvirusprecursorsynchronizingarrestDNAreplicationInhibitorinhibitorinhibit

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MedChemExpress (MCE) 只为有资质的科研机构、医药企业基于科学研究或药证申报的用途提供医药研发服务，不为任何个人或者非科研性质的、非用于药证申报使用等其他用途提供服务。

站点地图隐私声明

沪ICP备15051369号-4

上海工商

沪公网安备31011502019417

沪(浦)应急管危经许[2021]201709(QFYS)

营业执照（三证合一）

Thymidine (Synonyms: 胸苷; 胸腺嘧啶核苷; DThyd; NSC 21548)

Customer Review

Other Forms of Thymidine:

MCE 顾客使用本产品发表的 23 篇科研文献