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  3. Ac-LETD-AFC

Ac-LETD-AFC 是一种 caspase-8 荧光底物。Ac-LETD-AFC 可检测 caspase-8 荧光活性,用于肿瘤细胞凋亡和氧化应激代谢的研究。

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Custom Peptide Synthesis

Ac-LETD-AFC Chemical Structure

Ac-LETD-AFC Chemical Structure

CAS No. : 210345-02-1

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规格 价格 是否有货 数量
1 mg ¥1318
3 - 4 周
5 mg   询价  
10 mg   询价  

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Customer Review

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Ac-LETD-AFC is a caspase-8 fluorogenic substrate. Ac-LETD-AFC can measure caspase-8 fluorogenic activity and can be used for the research of cancer cell apoptosis and oxidative stress metabolism[1].

体外研究
(In Vitro)

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Caspase activity assay[1]:
1. Incubate the cells according to your normal protocol.
2. Wash cells once with PBS and centrifugate.
3. Resuspend cells in PBS at a concentration of 1× 107 cells/mL.
4. Prepare the standard reaction buffer which includ 100 μM caspase-8 substrate AC-LETD-AFC. Prepare correspounding standard reaction buffer according to your protocol.
5. Add 15 ul of the cells suspension to a microplate, and mixed with the appropriate peptide substrate dissolved in a standard reaction buffer.
6. Measure substrate cleavage with a microplate reader with excitation wavelength of 360 nm and emission at 460 nm.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

729.65

Formula

C31H38F3N5O12

CAS 号
运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

纯度 & 产品资料
参考文献
  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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产品名称:
Ac-LETD-AFC
目录号:
HY-P2620
需求量: