1. 抗体
  2. 一抗
  3. 单克隆抗体 重组抗体
  4. BACE 抗体 (YA1914)

BACE 抗体 (YA1914)

目录号: HY-P82169
COA 抗体使用指南 技术支持

BACE Antibody (YA1914) 是一个兔来源、无偶联标记、抗 BACE 的 IgG 单克隆抗体。

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规格 价格 是否有货 数量
10 μL ¥385 In-stock
50 μL ¥1000 In-stock
100 μL ¥1600 In-stock
250 μL   询价  

* Please select Quantity before adding items.

【WB: 蛋白质免疫印迹; IHC-P: 石蜡切片样本的免疫组织化学; IHC-F: 冰冻切片样本的免疫组织化学; ICC/IF: 细胞免疫荧光; IF-Tissue: 组织免疫荧光; mIHC: 多重荧光免疫组化; IP: 免疫沉淀; ChIP: 染色质免疫沉淀; FC: 流式细胞术; ELISA: 酶联免疫吸附试验】

  • 生物活性

  • 技术参数

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描述

BACE Antibody (YA1914) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to BACE.

研究背景

BACE1 蛋白在淀粉样前体蛋白 (APP) 的蛋白水解过程中发挥着关键作用。它在 A-β 肽序列的 N 末端(特别是 APP 的残基 671 和 672 之间)启动切割,从而产生 β 切割的可溶性 APP,并随后将其释放到细胞外。同时,BACE1 生成相应的细胞相关 C 末端片段,随后由 γ 分泌酶释放。除了其主要靶标外,BACE1 还对 CHL1 表现出切割活性,正如通过其切割该底物的相似性观察到的。

基因 ID
蛋白数据库
中文名
BACE 抗体 (YA1914)
同用名
BACE1; BACE; KIAA1149; Beta-secretase 1; Aspartyl protease 2; ASP2; Asp 2; Beta-site amyloid precursor protein cleaving enzyme 1; Beta-site APP cleaving enzyme 1; Memapsin-2; Membrane-associated aspartic protease 2
分子量

Predicted band size: 56 kDa; Observed band size: 68 kDa

纯度

Affinity Chromatography

偶联

Non-conjugated

修饰

Unmodified

RRID
研究领域

Neuroscience

产品类别

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

克隆性

Recombinant, Monoclonal

宿主

Rabbit

反应物种

Human, Mouse, Rat

推荐稀释比例

WB: 1/500-1/1000;IP: 1/40

  • Western blot analysis of extracts from Hela(lane 2(20ug) , NIH/3T3(lane 3(20ug) and HEK293(lane 4(20ug) using BACE1 Antibody (HY-P82169) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
应用

WB, IP

性状

液体

组分

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

保存条件 & 期限

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

运输条件

Shipping with blue ice.

同型

IgG

敏感性

Endogenous

免疫原

A synthesized peptide derived from human BACE1

数据库
文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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产品名称:
BACE Antibody (YA1914)
目录号:
HY-P82169
需求量: