Caspase-3 抗体 (YA557)

Caspase-3 Antibody (YA557) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Caspase-3.

CASP3 是一种硫醇蛋白酶，作为主要的效应 caspase 参与细胞凋亡的执行阶段。在被起始 caspases（如 CASP8、CASP9 或 CASP10）切割并激活后，通过催化多种蛋白质的切割来介导细胞凋亡的执行。在细胞凋亡开始时，它会在 '216-Asp-|-Gly-217' 键处蛋白水解切割 poly(ADP-ribose) polymerase PARP1。类似功能包括切割和激活 sterol regulatory element binding proteins（SREBPs）在碱性螺旋-环-螺旋亮氨酸拉链结构域和膜附着结构域之间。此外，CASP3 还切割并激活 caspase-6（CASP6）、caspase-7（CASP7）和 caspase-9（CASP9），同时切割并失活 interleukin-18（IL18）。它还参与 huntingtin 的切割，并通过 RET 切割触发交感神经元中的细胞粘附。在氧化应激反应中，CASP3 切割并抑制 serine/threonine-protein kinase AKT1。在病毒诱导的细胞凋亡过程中，CASP3 通过切割抗病毒蛋白 CGAS、IRF3 和 MAVS 来抑制 I 型干扰素的产生，从而防止细胞因子过度产生。此外，CASP3 还参与 pyroptosis，通过切割和激活 gasdermin-E（GSDME）发挥作用。它还能切割 XRCC4 和 phospholipid scramblase 蛋白 XKR4、XKR8 和 XKR9，促进凋亡细胞表面的 phosphatidylserine 暴露。最后，在 DIABLO/SMAC 抑制 BIRC6-caspase 结合后，CASP3 切割 BIRC6。

Free

P42574

Predicted band size: 32 kDa; Observed band size: 17 kDa

Protein A affinity purified.

Cytoplasm

Non-conjugated

Unmodified

AB_3102061

Cell Biology

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

WB: 1:500; ICC/IF: 1:50; IHC-P: 1:50; FC:1:20-1:50; IP: Use at an assay dependent concentration.

• 
  Western blot analysis of extracts from Hela (lane 2(20μg), Jurkat (lane 3(20μg) and HEK293 (lane 4(20μg) using Caspase-3(HY-P80046) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
• 
  Immunocytochemistry analysis of Hela cells labeling Caspase-3 with Caspase-3 Antibody (HY-P80046)at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Caspase-3 Antibody (HY-P80046) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

WB, ICC/IF, IHC-P, IP, FC

液体

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human Caspase-3.AA range:60-100.